For the Separation of Proteins,
Peptides, and Nucleic Acids
- High binding capacity and high flow rates
- Structure provides excellent rigidity and stability to pH and ionic strength changes, as well as to high temperature
- Easy cleaning with sodium hydroxide
- Scalable from research and development to manufacturing
- Separation of basic proteins (Cytochrome C, Lysozyme, etc.)
- Separtaion of human serum proteins and plant proteins such as lectins, glycoproteins, transferases, kinases, and trehalases
-
Separation of phosphate dependent proteins and exzymes as well as
DNA-dependent enzymes - Can be used to remove DNA and endotoxins
Average Particle Size
- 120 微米
Hydroxyapatite Content (Weight/Volume)
- 40%
Agarose (Weight/Volume)
- 4%
排阻限制
- > 5,000,000 Da
Capacity for Cytochrome C1
- > 7 毫克/毫升
Capacity for BSA2
- < 7 毫克/毫升
Working pH
- 5 - 13 (Note:Do not use < pH 4.0.)
Storage Temperature
- 2 - 30 ºC (36 - 86 °F)
- 2 - 8 ºC (36 - 46 °F) after opening
1Determined at 50% breakthrough using 5 mg/mL Cytochrome C diluted 50/50 in 10 mM PBS, pH 6.8
at 30 cm/h.
2Determined at 50% breakthrough using 1 mg/mL BSA diluted 50/50 in 10 mM PBS, pH 6.8 at 12.5 cm/h.
Separation of a Mixture of Ribonuclease and Phytohemagglutinins (PHA-EL)
Column:1.6 x 6.5 cm; Sample:1 mg of protein mixture composed of ribonuclease (MW 14,700) and PHA-ELs (Erythroagglutinating and lymphostimulating Phytohemagglutinin) (MW 128,000) from Phaseolus vulgaris, in 1 mL of 5 mM potassium phophate, pH 6.8; Elution gradient:5 mM to 500 mM potassium phosphate, pH 6.8; Flow rate:14.4 cm/h.