Blue Trisacryl M dye-affinity chromatography sorbent is used for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors.It is also used for albumin purification or albumin depletion in samples for proteomics research.
Blue Trisacryl M sorbent is based on Trisacryl GF2000 support, a macroporous non ionic synthetic polymer on which Cibacron blue 3GA is covalently immobilized through a stable six-carbon spacer arm to prevent leakage of the dye in normal working conditions.
- Unique multi-modal interaction mechanism
- Strong bond of dye-to-sorbent prevents leakage of the dye
- Multiple laboratory to production scale applications
Presentation and Storage
Blue Trisacryl M sorbent is available as ready-to-use labpacks suspended in 1 M sodium chloride with 20% (v/v) ethanol as bacteriostatic, or in drums for process-scale applications.
For fast screening laboratory applications, the sorbent is available in 1 mL AcroSep™ column (1.48 cm bed height x 0.94 cm bed diameter).This dark blue color coded column has threaded female luer lock inlets and rotating male luer locking hub outlets, and can be easily operated using a syringe or a pump.
Blue Trisacryl M Sorbent can be Used for the Purification of Many Proteins
Blue Trisacryl M sorbent is a group-specific adsorbent with affinity for a wide variety of enzymes.Some proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors (ADP, NADP).Other proteins like albumin or interferon bind by a combination of hydrophobic, electrostatic and pi-pi interactions.
Applications include :
- Enzymes which need NAD as cofactor (kinases, dehydrogenases, phosphatases…)
- Other enzymes (sulfatases, RNA polymerases, mono-oxygenases, oxydoreductases)
- Albumins from plasma
- Transferring from plasma
- α1-acid glycoprotein, α-fetoprotein, α1-proteinase inhibitor
- Serine proteases
- DNA antibodies
Recombinant vaccines purification processes
Example ApplicationsExample I. Separation of Human Plasma Proteins
Column:1.6 cm I.D. x 10 cm ; Buffer:0.05 M Tris-HCl, pH 8.8. Elution by an NaCl gradient from 0 to 3 M.
Example II. Automatic Separation of Human Albumin from Plasma by Step Elution
Column:2.5 cm I.D. x 6 cm; Adsorption buffer:0.05 M Tris-HCl, 0 5 M NaCI, pH 8; Albumin elution buffer:2.5 M NaCl in the same buffer; Regeneration solution:water-ethylene glycol mixture (50:50). —— UV absorbance at 280 nm. - — - lonic strength.- - - - pH.
Example III Depletion of Albumin From Human Plasma Using AcroSep™ Columns
L = Load, FT = Flowthrough, E = Elution.Albumin is bound to Blue Trisacryl M sorbent in 20 mM PBS buffer, pH 7.2 and eluted by an NaCl gradient from 0 to 3 M
Main Properties of Blue Trisacryl M Sorbent
|颗粒尺寸||40 - 80 微米|
|配基||Cibacron blue 3GA|
|Capacity for human albumin1||≥10 mg/mL|
|Thermal stability||2 to 121 °C|
|Working pH||4 至 10|
|清洁 pH||1 to 12 (short term)|
|Working pressure at 100 cm/hr||Up to 3 bar (45 psi)|
Chemical and Mechanical Stability
The chemical stability of Blue Trisacryl M sorbent is a function of the synthetic nature of Trisacryl matrix and the enhanced stability of the ligand coupling mechanism.The sorbent can be operated up to 3 bar backpressure while maintaining good flow rate properties.
The binding capacity of Blue Trisacryl M sorbent depends on the protein, the composition of the feedstock and parameters such as pH.Note that the capacity for a given protein may differ according to the animal species (for example, the binding capacity for bovine albumin is lower than for human albumin).
Ordering Information for Blue Trisacryl M Sorbent
|蓝色Trisacryl M 填料|
|Blue Trisacryl M AcroSep column|
|Blue Trisacryl M, 1 mL, Dark Blue 5/pkg||25896-C001|