Small-scale, single-use, stirred bioreactor
Highlights and Benefits
- Bench top unit with a maximum 13 L working volume, enabling to quickly develop processes at lower costs while keeping current lab infrastructure
- Flexible system offering several configurations to best meet the end user needs:single-use and/or classical probes, dynamic or static sparger, process control system or open architecture
- Superior mixing capabilities with highly reduced shear stress specially designed for suspension of microcarriers at high densities
- User friendly and smart process control system that can be operated in a 21 CFR Part 11 compliant environment (optional)
概述
The PadReactor Mini bioreactor is a scaled down version of the PadReactor bioreactor, making it ideal for feasibility studies, research, process development and small volume production of proteins, cells and viruses.Its innovative cube-shaped biocontainer design incorporates a highly efficient mixing paddle and a strong sparging device for a superior mixing capabilities as well as better gas transfer.These features make the PadReactor Mini system particularly adapted for the cultivation of suspension cells and adherent cells microcarriers.The PadReactor Mini platform consists of a single-use biocontainer fitted in a stainless steel holder and connected to the process controller.The system is designed to make the biocontainer installation fast and straightforward.On top of the biocontainer holder, the mechanical mixing head driven by a motor mounted on the system can be easily coupled to the paddle.A top cover secures the placement of the biocontainer assemblies as well as the pH and DO probes.A transparent front cover enables the user to easily observe the process during the culture and gives access to the sampling port, temperature sensor as well as single-use pH and DO probes.
The PadReactor Mini biocontainer is designed to be ready-touse.The platform integrates a process control system, including pumps and gas modules.The controller, compliant with GAMP 4 guidelines, ensures a reliable, accurate and fully documented control of critical cell culture parameters such as pH, DO, and temperature.In case the end user would still prefer to use the PadReactor system with its own process controller, the PadReactor system can be supplied in an open architecture configuration.
Contact your 颇尔 Life Sciences representative to discuss how the PadReactor Mini bioreactor can help you to quickly develop and optimize your cell culture process.
- Production of monoclonal antibodies in suspension cells
- Production of vaccines in adherent cells on microcarriers
- Growth of stem cells for cell therapy applications
Volume and Dimensions
Working Volume | 13 L |
Biocontainer Holder (w x d x h) | 306 x 296 x 664 mm |
Process Control System (w x d x h) | 408 x 474 x 711 mm |
PadReactor Mini Biocontainer and Holder
- Allegro™ TK8 film material:multi-layer with contact layer in ULDPE, gamma sterilizable, USP Class VI, fully ADCF (Animal Derived Component Free)
- The heating of the PadReactor Mini biocontainer is made via a heating blanket placed on the biocontainer holder.Temperature control is performed through pt100 probe
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Sparging device available in two configurations:
- Static and placed at the bottom of the single-use biocontainer
- Dynamic, coupled to paddle
- Multiports on top of the biocontainer:Gas IN/OUT; base addition, inoculum, Media IN, ports for classical DO and pH sensors
- Ports on the front side of the biocontainer:sampling, temperature, and single-use DO and pH sensors
- Ports on the bottom of the biocontainer:draining, O2 inlet (sparging device)
Process Control System
Startup Requirements
Gas Requirements | Pressure |
O2 | 2 bar |
CO2 | 2 bar |
N2 | 2 bar |
Air | 2 bar |
Power Requirements |
110/230 VAC 1300VA |
System Specifications
检测仪 | 描述 |
pH Probes |
Classical and/or Single-Use patches (pre-calibrated, USP Class VI) |
DO Probes |
Classical and/or Single-Use patches (pre-calibrated, USP Class VI) |
Temperature Probes | 1 pt 100 (50 mm, Ø 4 mm) |
泵 |
2 (base addition – 102R, Watson Marlow) [20 RPM] & Media IN/OUT (Easyload II, Watson Marlow) [0 – 300 RPM] |
Rotameters | 500 mL/min |
Mass Flowmeter | 1000 mL/min |
Control Parameters | 描述 |
pH 范围 |
Classical probes:0.0 – 14.0 pH, (± 0.01 pH) Single-use probes:6.0 – 9.0 pH, (± 0.1 pH) |
pH Control | NaOH addition/CO2 addition |
DO Range |
Classical probe:0% air – 500% air / 0% oxygen) – 100% (oxygen), (± 0.1%) Single-use probe:0% – 150% airsat (± 2%) |
DO Control | Air/O2/N2 addition |
温度范围 | 0 – 150 °C (± 0.1 °C) |
Temperature Control | pt 100 probe |
Speed Range | 0 – 100 RPM |
Gas Flow Rates – O2 | 0 – 1 L/min |
Gas Flow Rates – CO2 | 0 – 500 mL/min |
Gas Flow Rates – N2 | 0 – 500 mL/min |
Gas Flow Rates – Air | 0 – 500 mL/min |
Control System | 描述 |
System Compliance |
GAMP, 21 CFR Part 11 (option), CE/UL/CSA compliant Online and offline data collection |
Data Management | Minimum sampling frequency:1 minute Data export in various formats (Excell, ASCII, BMP) |
Data Display | Various graph types (line, bars, synoptic) |
Alarm | Available |
Part Number | 描述 |
740034 | PadReactor Mini holder (230 V – EU) |
740036 | PadReactor Mini holder (110 V – US) |
740041 | PadReactor Mini premium controller (230 V) |
740043 | PadReactor Mini premium controller (110 V) |
730362 | PadReactor Mini biocontainer, 16 L, static micro sparger, sterile |
730363 | PadReactor Mini biocontainer, 16 L, static macro sparger, sterile |
730364 | PadReactor Mini biocontainer, 16 L, dynamic micro sparger, sterile |
730365 | PadReactor Mini biocontainer, 16 L, dynamic macro sparger, sterile |