pall,颇尔,Micro-24 微型反应器系统,MRT-MICROSYS-M, MRT-CCSYS-M

Micro-24 微型反应器系统


pall,颇尔,Micro-24 微型反应器系统,MRT-MICROSYS-M, MRT-CCSYS-M
pall,颇尔,Micro-24 微型反应器系统

Micro-24 微型反应器系统
The 颇尔 Micro-24 MicroReactor system is a compact solution for efficient cell line evaluation and process development.With options for a variety of culture conditions including aerobic & anaerobic microbial fermentation, mammalian & insect cell culture, the Micro-24 System offers the most flexible operating conditions available.The Micro-24 MicroReactor system runs up to 24 simultaneous bioreactor experiments with independent control and monitoring of each reactor's gas supply, temperature and pH.It is designed for easy set-up and operation using single-use cassettes and closures.


  • Single-use MicroReactor Cassettes:
    • 24 reactors per cassette
    • pH and DO sensors pre-calibrated
    • Irradiated and ready to use
    • Working volume is 3 to 7 mL
  • Independent control of each MicroReactor:
    • Dissolved oxygen
    • pH
    • Temperature
  • System-level control of agitation
  • Real-time monitoring and control

The Micro-24 MicroReactor Instrument

The Micro-24 MicroReactor features an integrated vacuum system to secure the single-use cassette during operation.Efficient mixing is achieved through an orbiting baseplate .Mixing conditions can be varied to support processes from shear sensitive stem cells to high density microbial.Each reactor in the single use cassette features the following sensing and control features:

  • Gas injection port for up to three control gases
  • Individual heater for temperature control
  • Individual thermocouple for temperature monitoring
  • pH optical sensor
  • DO optical sensor

For More Information

For more information on the Micro-24 MicroReactor, or to arrange a call with a 颇尔 Process Development Specialist, please contact us


Product Applications

颇尔 Micro-24 MicroReactor systems are in use worldwide to grow a wide range of cell types under highly diverse conditions.Our systems offer significant advantages over shake flask screening for fast growing organisms and high output processes In addition to the highly aerobic processes common to industrial fermentation, 颇尔 MicroReactor systems are also being used to study environmentally interesting organisms under controlled anaerobic and micro-aerobic conditions.The Micro-24 MicroReactor system also excels in the execution of longer term mammalian and insect cell culture.Efficient mixing is delivered in a low shear environment providing high throughput capabilities for controlled process development.The superior mixing provided by the Micro-24 MicroReactor system allows researchers the flexibility to optimize processes for adherent cells on microcarriers as well as suspension cell culture.

Clone Selection

For most companies, clone production easily outpaces the available resources for data driven evaluation.The 颇尔 Micro-24 MicroReactor technology offers the ability to screen hundreds to thousands of clones in controlled and monitored reactors.Thorough evaluation in a controlled reactor dramatically enhances the chances of selecting the best clones and minimizes the risks of unexpected performance during scale-up.

Media development and optimization

Rapidly screen a wide range of media and components in relevant conditions.Determine critical media components, trace nutrient fate using costly labeled substrates, and screen multiple microcarriers in parallel.Process control with minimal downtime ensures that studies are rapid and decisive.

Parametric optimization

From characterization of growth and specific productivity across a range of conditions to studying the timing of induction or temperature shifts on final productivity, 颇尔 Micro-24 MicroReactors can provide critical process insights faster and earlier in process development.The parallel nature of the reaction vessels make the system ideal for DoE studies and multivariate analysis.


There are thousands of uses for reproducible production of milligram quantities of target proteins.Common examples include characterization, crystallography, proteomic studies, or the generation of affinity tags.The small format and capability of the Micro-24 MicroBioreactor system makes it uniquely suited to generate large numbers of small protein samples.

Typical Application Organisms

  • 大肠杆菌
  • Pseudomonas fluorescens
  • Saccharomyces cerevisiae
  • Pichia pastoris
  • Bacillus subtilis
  • Shewanella oneidensis MR-1
  • Aspergillus nidulans
  • Trichoderma reesei
  • Aspergillus oryzae
  • Cell-free expression systems
  • Mammalian suspension cultures such as CHO
  • Human T-cells
  • Mouse and Human stem cell lines
  • Blood cells
  • Vero cells
  • Insect cell lines

For More Information

For more information on the Micro-24 MicroReactor, or to arrange a call with a 颇尔 Process Development Specialist, please contact us








Micro-24™ MicroReactor System Specification

Dissolved Oxygen  
Air/oxygen feed 0 - 20 sccm
DO setpoint range 0 - 100% air saturation
DO accuracy ± 5% at 0% air saturation, ± 10% at 100% air saturation


Standard pH sensor setpoint range 6.0 - 8.0
Low pH sensor setpoint range     3.5 – 5.5    
pH sensitivity ± 0.1 pH unit 


Temperature (per Well)  
T setpoint range 18 °C - 45 °C
T sensitivity ± 0.1 °C
Maximum achievable T gradient *      

10 °C   

*appropriate set-up required for gradient operation


Shaker 0-800 rpm programmable; 5mm orbital throw
Power requirements 110V , 5 A  service,
or 220 V, 2 A service
Air requirements

Clean Dry Instrument Air >0.000472 cms (1.0 CFM), 4.5-7.5 Bar (65-110 psi),  Control Gases 1-2.1 Bar (15-30 psi), ¼ in.  or 6mm  fittings   

尺寸 480 mm x 280 mm x 432 mm (w x h x d)
System Weight 22.5 Kilograms


For More Information

For more information on the Micro-24 MicroReactor, or to arrange a call with a 颇尔 Process Development Specialist, please contact us


24-Well Cassette Options and Details

Three cassette designs provide a variety of process conditions. 

  • Standard [REG]
  • Baffled [BFL]
  • Central Vent [PRC]

Polycarbonate, irradiated by e-beam

颇尔 Micro-24 MicroReactor Microbial Cassette:Individual Reactor Detail

Schematic diagram illustrating the functioning of the Micro-24 MicroReactor microbial cassettes.Each of the three gas feeds is controlled individually for each reactor.In microbial applications, pH is controlled with either dilute ammonia or carbon dioxide.Oxygen control is achieved with either nitrogen or oxygen.Gases are sparged directly into the culture medium through the gas-permeable hydrophobic membrane at the bottom of each reactor well.


颇尔 Micro-24 MicroReactor Cell Culture Cassette:Individual Reactor Detail

Schematic diagram illustrating the functioning of the Micro-24 MicroReactor cell culture cassette.Up to 3 gas feeds can be individually controlled per reactor. In this design, gases pass through the sterilizing membrane into the vent tube bypassing the media.This headspace gassing design allows control gases to diffuse into the culture medium during orbital agitation eliminating the need for antifoam.

Four Options for Well Closures

Type A:Single-use with neoprene check valve and sterile membrane barrier

Type D:Single-use with sterile membrane barrier

Type E:Autoclavable, re-usable, with stainless steel check valve, no sterile membrane barrier

Irradiated Tape Closure:Single-use, good for high gas exchange conditions, short experiment durations

Recommended cassette/closure combination for specific applications

Culture Type 细菌 Yeast
Cell Density 全部 OD600 < 40 OD600 > 40 全部 OD600 < 30 OD600 > 30
O2 Conditions Anaerobic Aerobic Anaerobic Aerobic
pH Conditions 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0
Cap TypeA/E TypeA/E TypeD TypeA/E TypeA/E TypeD
Gas1 CO2 (optional)
Gas2 NH3/N2 NH3/Air  NH3/N2 NH3/Air
Gas3 N2 O2 N2 O2

Culture Type Filamentous Fungi 细胞培养
Cell Density 全部 全部
O2 Conditions Anaerobic Aerobic < 50% DO > 50% DO
pH Conditions 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 6.0 - 8.0
Cap TypeA/E TypeD TypeA/E TypeD
Gas1 CO2 (optional) 20-50% CO2/N2
Gas2 NH3/N2 NH3/Air 5-8% CO2/N2
Gas3 N2 O2 40 - 60% O2/N2


Rapid experimental set-up

For a quotation or to place an order for a Micro-24 MicroReactor system, please contact 上海羽令 so that we may review the requirements for installation at your site.颇尔 strongly recommends a brief post-installation training program for the operator, delivered at the operator site by a 颇尔 technical specialist.


颇尔 Micro-24 MicroReactor Part Numbers


Part Number 描述
MRT-MICROSYS-M Complete Microbial System - Metric
MRT-CCSYS-M Complete Cell Culture System-Metric


Part Number 描述
MRT-M24-M Micro-24 MicroReactor, metric (EU/Asia) 
MRT-CMP  Laptop Controller w/Micro-24 MicroReactor software 
MRT-APV-3M Ammonia Pressure Vessel, metric
MRT-BOX-1 Reusable ABS Shipping Container with custom foam 
MRT-KIT-U Micro-24 instrument installation kit
 MRT-GMM-1 Micro-24 Gas Micro Mixer Accessory


Part Number 描述
MRT-REG-2CS  Case of 6 Standard Cassettes 
MRT-BFL-2CS  Case of 6 Baffled Cassettes - for high viscosity applications
MRT-PRC-2CS Case of 6 Cell Culture Cassettes 
MRT-REG-LOW-CS Case of 6 Low pH Standard Cassettes
MRT-BFL-LOW-CS Case of 6 Low pH Baffle  Cassettes – for high viscosity applications
MRT-PRC-LOW-CS Case of 6 Low pH Cell Culture Cassettes
MRT-TPE-1 Set of 6 sterile sealing tapes 
MRT-CAP-A6  Case of 6x24 Type A Caps
MRT-CAP-D6  Case of 6x24 Type D Caps
MRT-CAP-E24  Set of 24 Type E Caps - autoclavable


Part Number 描述
PTS02MRTSVCINST Micro-24 Installation Service
PTS02MRTSVCTRN Micro-24 Start-up Training, per day
PTS02MRTSVCRNT Micro-24 Rental, 4 weeks


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