特点
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Single-use MicroReactor Cassettes:
- 24 reactors per cassette
- pH and DO sensors pre-calibrated
- Irradiated and ready to use
- Working volume is 3 to 7 mL
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Independent control of each MicroReactor:
- Dissolved oxygen
- pH
- Temperature
- System-level control of agitation
- Real-time monitoring and control
The Micro-24 MicroReactor Instrument
The Micro-24 MicroReactor features an integrated vacuum system to secure the single-use cassette during operation.Efficient mixing is achieved through an orbiting baseplate .Mixing conditions can be varied to support processes from shear sensitive stem cells to high density microbial.Each reactor in the single use cassette features the following sensing and control features:
- Gas injection port for up to three control gases
- Individual heater for temperature control
- Individual thermocouple for temperature monitoring
- pH optical sensor
- DO optical sensor
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a 颇尔 Process Development Specialist, please contact us
Product Applications
颇尔 Micro-24 MicroReactor systems are in use worldwide to grow a wide range of cell types under highly diverse conditions.Our systems offer significant advantages over shake flask screening for fast growing organisms and high output processes In addition to the highly aerobic processes common to industrial fermentation, 颇尔 MicroReactor systems are also being used to study environmentally interesting organisms under controlled anaerobic and micro-aerobic conditions.The Micro-24 MicroReactor system also excels in the execution of longer term mammalian and insect cell culture.Efficient mixing is delivered in a low shear environment providing high throughput capabilities for controlled process development.The superior mixing provided by the Micro-24 MicroReactor system allows researchers the flexibility to optimize processes for adherent cells on microcarriers as well as suspension cell culture.Clone Selection
For most companies, clone production easily outpaces the available resources for data driven evaluation.The 颇尔 Micro-24 MicroReactor technology offers the ability to screen hundreds to thousands of clones in controlled and monitored reactors.Thorough evaluation in a controlled reactor dramatically enhances the chances of selecting the best clones and minimizes the risks of unexpected performance during scale-up.
Media development and optimization
Rapidly screen a wide range of media and components in relevant conditions.Determine critical media components, trace nutrient fate using costly labeled substrates, and screen multiple microcarriers in parallel.Process control with minimal downtime ensures that studies are rapid and decisive.
Parametric optimization
From characterization of growth and specific productivity across a range of conditions to studying the timing of induction or temperature shifts on final productivity, 颇尔 Micro-24 MicroReactors can provide critical process insights faster and earlier in process development.The parallel nature of the reaction vessels make the system ideal for DoE studies and multivariate analysis.
Microproduction
There are thousands of uses for reproducible production of milligram quantities of target proteins.Common examples include characterization, crystallography, proteomic studies, or the generation of affinity tags.The small format and capability of the Micro-24 MicroBioreactor system makes it uniquely suited to generate large numbers of small protein samples.
Typical Application Organisms
- 大肠杆菌
- Pseudomonas fluorescens
- Saccharomyces cerevisiae
- Pichia pastoris
- Bacillus subtilis
- Shewanella oneidensis MR-1
- Aspergillus nidulans
- Trichoderma reesei
- Aspergillus oryzae
- Cell-free expression systems
- Mammalian suspension cultures such as CHO
- Human T-cells
- Mouse and Human stem cell lines
- Blood cells
- Vero cells
- Insect cell lines
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a 颇尔 Process Development Specialist, please contact us
Micro-24™ MicroReactor System Specification
| Dissolved Oxygen | |
| Air/oxygen feed | 0 - 20 sccm |
| DO setpoint range | 0 - 100% air saturation |
| DO accuracy | ± 5% at 0% air saturation, ± 10% at 100% air saturation |
| pH | |
| Standard pH sensor setpoint range | 6.0 - 8.0 |
| Low pH sensor setpoint range | 3.5 – 5.5 |
| pH sensitivity | ± 0.1 pH unit |
| Temperature (per Well) | |
| T setpoint range | 18 °C - 45 °C |
| T sensitivity | ± 0.1 °C |
| Maximum achievable T gradient * |
10 °C |
*appropriate set-up required for gradient operation
| 系统 | |
| Shaker | 0-800 rpm programmable; 5mm orbital throw |
| Power requirements |
110V , 5 A service, or 220 V, 2 A service |
| Air requirements |
Clean Dry Instrument Air >0.000472 cms (1.0 CFM), 4.5-7.5 Bar (65-110 psi), Control Gases 1-2.1 Bar (15-30 psi), ¼ in. or 6mm fittings |
| 尺寸 | 480 mm x 280 mm x 432 mm (w x h x d) |
| System Weight | 22.5 Kilograms |
For More Information
For more information on the Micro-24 MicroReactor, or to arrange a call with a 颇尔 Process Development Specialist, please contact us
24-Well Cassette Options and Details
Three cassette designs provide a variety of process conditions.
- Standard [REG]
- Baffled [BFL]
- Central Vent [PRC]
Polycarbonate, irradiated by e-beam
颇尔 Micro-24 MicroReactor Microbial Cassette:Individual Reactor Detail
Schematic diagram illustrating the functioning of the Micro-24 MicroReactor microbial cassettes.Each of the three gas feeds is controlled individually for each reactor.In microbial applications, pH is controlled with either dilute ammonia or carbon dioxide.Oxygen control is achieved with either nitrogen or oxygen.Gases are sparged directly into the culture medium through the gas-permeable hydrophobic membrane at the bottom of each reactor well.
颇尔 Micro-24 MicroReactor Cell Culture Cassette:Individual Reactor Detail
Schematic diagram illustrating the functioning of the Micro-24 MicroReactor cell culture cassette.Up to 3 gas feeds can be individually controlled per reactor. In this design, gases pass through the sterilizing membrane into the vent tube bypassing the media.This headspace gassing design allows control gases to diffuse into the culture medium during orbital agitation eliminating the need for antifoam.
Four Options for Well Closures
Type A:Single-use with neoprene check valve and sterile membrane barrier
Type D:Single-use with sterile membrane barrier
Type E:Autoclavable, re-usable, with stainless steel check valve, no sterile membrane barrier
Irradiated Tape Closure:Single-use, good for high gas exchange conditions, short experiment durations
Recommended cassette/closure combination for specific applications
| Culture Type | 细菌 | Yeast | ||||
| Cell Density | 全部 | OD600 < 40 | OD600 > 40 | 全部 | OD600 < 30 | OD600 > 30 |
| O2 Conditions | Anaerobic | Aerobic | Anaerobic | Aerobic | ||
| pH Conditions | 3.5 - 5.5 / 6.0 - 8.0 | 3.5 - 5.5 / 6.0 - 8.0 | 3.5 - 5.5 / 6.0 - 8.0 | 3.5 - 5.5 / 6.0 - 8.0 | 3.5 - 5.5 / 6.0 - 8.0 | 3.5 - 5.5 / 6.0 - 8.0 |
| Cassette | PRCLOW / PRC | PRCLOW / PRC | REGLOW / REG | PRCLOW / PRC | PRCLOW / PRC | REGLOW / REG |
| Cap | TypeA/E | TypeA/E | TypeD | TypeA/E | TypeA/E | TypeD |
| Gas1 | CO2 (optional) | |||||
| Gas2 | NH3/N2 | NH3/Air | NH3/N2 | NH3/Air | ||
| Gas3 | N2 | O2 | N2 | O2 | ||
| Culture Type | Filamentous Fungi | 细胞培养 | ||
| Cell Density | 全部 | 全部 | ||
| O2 Conditions | Anaerobic | Aerobic | < 50% DO | > 50% DO |
| pH Conditions | 3.5 - 5.5 / 6.0 - 8.0 | 3.5 - 5.5 / 6.0 - 8.0 | 6.0 - 8.0 | |
| Cassette | BFLLOW / BFL | BFLLOW / BFL | PRC | |
| Cap | TypeA/E | TypeD | TypeA/E | TypeD |
| Gas1 | CO2 (optional) | 20-50% CO2/N2 | ||
| Gas2 | NH3/N2 | NH3/Air | 5-8% CO2/N2 | |
| Gas3 | N2 | O2 | 40 - 60% O2/N2 | |
CONFIGURATION SCREEN
Rapid experimental set-up
颇尔 Micro-24 MicroReactor Part Numbers
系统
| Part Number | 描述 |
| MRT-MICROSYS-M | Complete Microbial System - Metric |
| MRT-CCSYS-M | Complete Cell Culture System-Metric |
硬件
| Part Number | 描述 |
| MRT-M24-M | Micro-24 MicroReactor, metric (EU/Asia) |
| MRT-CMP | Laptop Controller w/Micro-24 MicroReactor software |
| MRT-APV-3M | Ammonia Pressure Vessel, metric |
| MRT-BOX-1 | Reusable ABS Shipping Container with custom foam |
| MRT-KIT-U | Micro-24 instrument installation kit |
| MRT-GMM-1 | Micro-24 Gas Micro Mixer Accessory |
Consumables
| Part Number | 描述 |
| MRT-REG-2CS | Case of 6 Standard Cassettes |
| MRT-BFL-2CS | Case of 6 Baffled Cassettes - for high viscosity applications |
| MRT-PRC-2CS | Case of 6 Cell Culture Cassettes |
| MRT-REG-LOW-CS | Case of 6 Low pH Standard Cassettes |
| MRT-BFL-LOW-CS | Case of 6 Low pH Baffle Cassettes – for high viscosity applications |
| MRT-PRC-LOW-CS | Case of 6 Low pH Cell Culture Cassettes |
| MRT-TPE-1 | Set of 6 sterile sealing tapes |
| MRT-CAP-A6 | Case of 6x24 Type A Caps |
| MRT-CAP-D6 | Case of 6x24 Type D Caps |
| MRT-CAP-E24 | Set of 24 Type E Caps - autoclavable |
服务
| Part Number | 描述 |
| PTS02MRTSVCINST | Micro-24 Installation Service |
| PTS02MRTSVCTRN | Micro-24 Start-up Training, per day |
| PTS02MRTSVCRNT | Micro-24 Rental, 4 weeks |
