Rapid selectivity screening of HyperCel STAR AX anion exchanger.
- Convenient – Ready-to-use 1 mL and 5 mL prepacked columns.
- Easy to use – Direct connection to commonly-used laboratory chromatography systems such as ÄKTA* systems.
- Efficient – High packing efficiency (≥ 2,500 plates/meter).
- Consistent – Screen HyperCel STAR AX sorbent under reliable and reproducible conditions, and guarantee performance run after run.
"Salt-Tolerant" Anion Exchange Chromatography Sorbent
HyperCel STAR AX sorbent is composed of a rigid cellulose matrix that has excellent flow properties and generates low backpressure.它可提供：
- High dynamic binding capacity at short residence time
- Use in either capture, intermediate or polishing steps
- High dynamic binding capacity is maintained up to 15 mS/cm conductivity
- Direct scale up to pilot or production scale columns
*ÄKTA is a trademark of GE Healthcare companies.
Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate separation conditions.Once the separation conditions are selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column.
可以将两个或两个以上柱串联起来以增加柱高，并更真实地模拟中试规模或按比例缩减规模应用的现实条件。Further scale up can be achieved with minimal re-optimization by packing 颇尔 LRC empty glass columns.
HyperCel STAR AX Anion Exchange Chromatography Sorbents
- Native and recombinant proteins
- 疫苗
- Monoclonal and polyclonal antibodies
- 血液制品
- 生物制药
| 填料 | |
| Volume | 1 mL (5 mm I.D. x 50 mm) or 5 mL (8 mm I.D. x 100 mm) |
| Average particle size | 60 - 90 微米 |
| Dynamic binding capacity1 | >100 mg BSA/mL within pH range 7.5 – 8.0 (conductivity 2 – 15 mS/cm) |
| 配基 | 伯胺 |
| Storage solution | 30% isopropanol, 100 mM sodium phosphate, pH 4.3 |
| Working pH | Binding:pH 7.0 – 8.5; Elution:pH 3.0 – 8.5 |
| 清洁 pH | 1 – 14 |
| Working pressure2 | < 0.5 bar (7 psi) for 1 mL column; < 1 bar (14 psi) for 5 mL column |
| Column | |
| Outer dimensions | 10 x 100 mm for 1 mL column or 11.5 x 140 mm for 5 mL column |
| Materials of construction |
Body and end caps:Molded PP (Polypropylene) 17 µm frit:PP / PE (Polypropylene / Polyethylene) |
| Connections3 | Built-in 10-32 fittings |
1 Determined at 10% breakthrough, using 5 mg/mL bovine serum albumin (BSA) in 50 mM Tris-HCl, pH 8.5 at 2 minute residence time (RT).Conductivity 2 – 15 mS/cm by supplementation of NaCl.
2 Pressure at 600 cm/hr equivalent to:2 mL/min for 1 mL column, 5 mL/min for 5 mL column, in 0.1 M NaCl.
3 For HPLC/MPLC/ÄKTA* systems:direct connection.For systems equipped with ¼–28 or M6 connections:use a standard 10–32 male / ¼–28 or M6 female adaptor (not supplied).
*ÄKTA is a trademark of GE Healthcare companies.
| 描述 | Part Number |
| HyperCel STAR AX PRC Prepacked Columns | |
| PRC Column 5x50 HyperCel STAR AX, 1 mL | PRCSTARAX1ML |
| PRC Column 8x100 HyperCel STAR AX, 5 mL | PRCSTARAX5ML |
| HyperCel STAR AX 填料 | |
| 5 毫升 | 20197-018 |
| 25 毫升 | 20197-026 |
| 100 毫升 | 20197-032 |
| 1 L | 20197-046 |
| 5 L | 20197-058 |
| 10 L | 20197-064 |
