Unique Sorbent Designed to Eliminate Detergent from Biological Fluids
- High dynamic binding capacity for many different detergents.
- High recovery for proteins.
- High adsorption capacity for small, hydrophobic molecules.
- Stable in acid, polar organic, and oxidizing solutions.
- Composed of silica beads in which the pore volume is filled with a three-dimensional crosslinked hydrophobic polymer.The small pore size, particle size distribution (40 to 100 μm), and hydrophobic nature of the chemical groups make SDR HyperD sorbent ideal for detergent removal.
- Binds detergent molecules typically used in viral inactivation processes [i.e., Tri-n-Butyl Phosphate (TnBP) and Tritons X-100]
- Removal of both ionic and zwitter ionic detergents from biological solutions
Average Particle Size
- 70 微米
Nature of Polymer
- Hydrophobic, long aliphatic chains bind solvents
Recommended Residence Time
- 5 - 15 min
Binding Capacity for Triton X-100
- 60 - 80 mg/mL1
Working pH
- 2 - 12
Pressure Resistance
- 70 bar (7,000 kPa, 1,000 psi)
Storage Temperature
- 2 - 30 ºC (36 - 86 ºF)
- 2 - 8 ºC (36 - 46 ºF) after opening
- Do not freeze
1Determined using 5 mg/mL Triton X-100 in PBS, pH 7.4, 10% breakthrough, 300 cm/h.
SDR HyperD Detergent Removal:Spin Column Format (< 0.2 mL Volume)
| Detergent | Removal Efficiency from a Protein Sample1 | ||
| Volume (Wt./v) | + 1% | + 5% | + 10% |
| ASB-14 | > 99% | > 99% | 80-90% |
| ASB-14 + 6M urea | > 99% | 80-90% | NR |
| CHAPS | > 99% | > 99% | > 99% |
| CHAPS + 6M urea | > 99% | > 99% | 80-90% |
| SDS | > 99% | > 99% | > 99% |
| SDS + 0.1 M NaCl | > 99% | > 99% | > 99% |
110.2 mL of 5 mg/mL BSA in the presence of detergents (Wt./v). Bio-Rad dye binding assay used to follow removal of detergents.NR denotes “Not Recommended” as this detergent loading would exceed the column capacity.
Using a rapid spin column format, the binding capacity of SDR for detergents is still very high.In all cases, > 99% of detergent removed from 0.2 mL of a 1% solution.
- 采用 SDR HyperD 去污剂清除填料的 AcroSep 层析柱
