Preparative Sorbents for the Purification
of Biomolecules by Charge
- High binding capacity.“Gel-in-a-shell” design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity.
- High flow rates.Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product.This mechanism of mass transfer (known as “enhanced diffusion”) allows the sorbents to operate free of constraints.
- Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules.
- Does not shrink or swell in response to changes in pH, ionic strength, or flow rate.
- Rigid, non-compressible sorbents are easy to pack.
- Easy cleaning with sodium hydroxide.
- Scalable from research and development to manufacturing.
- Direct capture of biomolecules from a variety of feedstocks
- Purification of polypeptides, IgG, and albumin
- Large-scale purifications
- Purification of monoclonal antibodies from ascites or cell culture
- Process polishing steps
|Type of Ceramic HyperD F Resin||Q||S||DEAE||CM|
|Particle Size (µm)||~50||~50||~50||~50|
Dynamic Binding Capacity (mg/mL)
10% Breakthrough at 200 cm/h
|BSA 851||Lysozyme 852||BSA 851||IgG 603|
|Amount of Ionic Groups (µeq/mL)||250||150||200||250 - 400|
|Working pH||2 - 12||2 - 12||2 - 12||2 - 12|
|清洁 pH||1 - 14||1 - 14||1 - 14||1 - 14|
Volume Changes Due to
pH and Ionic Strength
|Pressure Resistance||20 grade:200 bar (20,000 kPa, 2,901 psi)||F grade:> 70 bar (7,000 kPa, 1,015 psi)|
2 - 30 °C (36 - 56 °F)
1Sample:5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
2Sample:5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5
3Sample:5 mg/mL Human IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7
Dynamic Binding Capacity of Ceramic HyperD F Ion Exchange Resin Packed in 1 mL Chromatography Glass Columns at a Range of Flow Rates (Linear Velocities)
|Dynamic Binding Capacity (mg/mL)1|
|介质||1 mL/min (258 cm/h)||5 mL/min (1290 cm/h)||10 mL/min|
|HyperD F DEAE||101.5||87.5||77.5|
|HyperD F S||80.5||61.5||53.5|
|HyperD F CM||108.0||87.5||73.5|
1Dynamic binding capacity measured by breakthrough curve analysis at 10% of media saturation; a 1 mL volume column of ion exchange resin was packed and equilibrated with 25 mM Tris HCl pH 8.5 (anion ion exchange) or 10 mM MES-NaOH pH 5.5 (cation ion exchange) at the flow rates of 1, 5, or 10 mL/min.For anion ion exchange, 5 mg/mL BSA in the above buffer was then pumped onto the column until a break through in absorbance at 280 nm was seen.The flow was continued until a plateau in absorbance was achieved corresponding to 100% protein feed.Dynamic binding capacity was then calculated at 10% of the plateau value, correcting for any "dead volume" in the system and expressed as mg BSA/mL media volume.For cation ion exchange, 5 mg/mL lysozyme was used to test these resins in a similar manner to the anion ion exchange media.