Preparative Sorbents for the Purification
of Biomolecules by Charge
- High binding capacity.“Gel-in-a-shell” design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity.
- High flow rates.Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product.This mechanism of mass transfer (known as “enhanced diffusion”) allows the sorbents to operate free of constraints.
- Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules.
- Does not shrink or swell in response to changes in pH, ionic strength, or flow rate.
- Rigid, non-compressible sorbents are easy to pack.
- Easy cleaning with sodium hydroxide.
- Scalable from research and development to manufacturing.
- Direct capture of biomolecules from a variety of feedstocks
- Purification of polypeptides, IgG, and albumin
- Large-scale purifications
- Purification of monoclonal antibodies from ascites or cell culture
- 质粒纯化
- Process polishing steps
| Type of Ceramic HyperD F Resin | Q | S | DEAE | CM |
| Grade | F | F | F | F |
| Particle Size (µm) | ~50 | ~50 | ~50 | ~50 |
|
Dynamic Binding Capacity (mg/mL) 10% Breakthrough at 200 cm/h |
BSA 851 | Lysozyme 852 | BSA 851 | IgG 603 |
| Amount of Ionic Groups (µeq/mL) | 250 | 150 | 200 | 250 - 400 |
| Working pH | 2 - 12 | 2 - 12 | 2 - 12 | 2 - 12 |
| 清洁 pH | 1 - 14 | 1 - 14 | 1 - 14 | 1 - 14 |
|
Volume Changes Due to pH and Ionic Strength |
Non -compressible |
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| Pressure Resistance | 20 grade:200 bar (20,000 kPa, 2,901 psi) | F grade:> 70 bar (7,000 kPa, 1,015 psi) | ||
| Storage Temperature |
2 - 30 °C (36 - 56 °F) |
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1Sample:5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
2Sample:5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5
3Sample:5 mg/mL Human IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7
Dynamic Binding Capacity of Ceramic HyperD F Ion Exchange Resin Packed in 1 mL Chromatography Glass Columns at a Range of Flow Rates (Linear Velocities)
| Dynamic Binding Capacity (mg/mL)1 | |||
| 介质 | 1 mL/min (258 cm/h) | 5 mL/min (1290 cm/h) | 10 mL/min |
| HyperD F DEAE | 101.5 | 87.5 | 77.5 |
| HyperD F S | 80.5 | 61.5 | 53.5 |
| HyperD F CM | 108.0 | 87.5 | 73.5 |
1Dynamic binding capacity measured by breakthrough curve analysis at 10% of media saturation; a 1 mL volume column of ion exchange resin was packed and equilibrated with 25 mM Tris HCl pH 8.5 (anion ion exchange) or 10 mM MES-NaOH pH 5.5 (cation ion exchange) at the flow rates of 1, 5, or 10 mL/min.For anion ion exchange, 5 mg/mL BSA in the above buffer was then pumped onto the column until a break through in absorbance at 280 nm was seen.The flow was continued until a plateau in absorbance was achieved corresponding to 100% protein feed.Dynamic binding capacity was then calculated at 10% of the plateau value, correcting for any "dead volume" in the system and expressed as mg BSA/mL media volume.For cation ion exchange, 5 mg/mL lysozyme was used to test these resins in a similar manner to the anion ion exchange media.
