pall,颇尔,HyperD F 离子交换填料(Q、S、DEAE 和 CM),20067-C001, 20066-C001, 20062-C001, 20050-C001, 20066-031, 20066-023, 20066-015, 20062-030, 20062-022, 20062-014, 20067-039, 20067-021, 20067-013, 20050-035, 200

产品名称:
HyperD F 离子交换填料(Q、S、DEAE 和 CM)
产品型号:
20067-C001, 20066-C001, 20062-C001, 20050-C001, 20066-031, 20066-023, 20066-015, 20062-030, 20062-022, 20062-014, 20067-039, 20067-021, 20067-013, 20050-035, 20050-027, 20050-019
产品描述:

产品介绍

pall,颇尔,HyperD F 离子交换填料(Q、S、DEAE 和 CM),20067-C001, 20066-C001, 20062-C001, 20050-C001, 20066-031, 20066-023, 20066-015, 20062-030, 20062-022, 20062-014, 20067-039, 20067-021, 20067-013, 20050-035, 200
pall,颇尔,HyperD F 离子交换填料(Q、S、DEAE 和 CM)

HyperD F 离子交换<a href='http://pall.net.cn/' target='_blank'>填料</a>(Q、S、DEAE 和 CM)

Preparative Sorbents for the Purification
of Biomolecules by Charge

  • High binding capacity.“Gel-in-a-shell” design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity.
  • High flow rates.Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product.This mechanism of mass transfer (known as “enhanced diffusion”) allows the sorbents to operate free of constraints.
  • Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules.
  • Does not shrink or swell in response to changes in pH, ionic strength, or flow rate.
  • Rigid, non-compressible sorbents are easy to pack.
  • Easy cleaning with sodium hydroxide.
  • Scalable from research and development to manufacturing.

 

 
 
  • Direct capture of biomolecules from a variety of feedstocks
  • Purification of polypeptides, IgG, and albumin
  • Large-scale purifications
  • Purification of monoclonal antibodies from ascites or cell culture
  • 质粒纯化
  • Process polishing steps
 
 
 
Type of Ceramic HyperD F Resin Q S DEAE CM
Grade F F F F
Particle Size (µm) ~50 ~50 ~50 ~50
Dynamic Binding Capacity (mg/mL)
10% Breakthrough at 200 cm/h
BSA 851 Lysozyme 852 BSA 851 IgG 603
Amount of Ionic Groups (µeq/mL) 250 150 200 250 - 400
Working pH 2 - 12 2 - 12 2 - 12 2 - 12
清洁 pH 1 - 14 1 - 14 1 - 14 1 - 14
Volume Changes Due to
pH and Ionic Strength

Non -compressible

Pressure Resistance 20 grade:200 bar (20,000 kPa, 2,901 psi) F grade:> 70 bar (7,000 kPa, 1,015 psi)
Storage Temperature

2 - 30 °C (36 - 56 °F)
2 - 8 °C (36 - 46°F) after opening

 

 

1Sample:5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
2Sample:5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5
3Sample:5 mg/mL Human IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7
 
 

Dynamic Binding Capacity of Ceramic HyperD F Ion Exchange Resin Packed in 1 mL Chromatography Glass Columns at a Range of Flow Rates (Linear Velocities)

 
  Dynamic Binding Capacity (mg/mL)1
介质 1 mL/min (258 cm/h) 5 mL/min (1290 cm/h) 10 mL/min
HyperD F DEAE  101.5 87.5 77.5
HyperD F S 80.5 61.5 53.5
HyperD F CM  108.0 87.5 73.5
 
1Dynamic binding capacity measured by breakthrough curve analysis at 10% of media saturation; a 1 mL volume column of ion exchange resin was packed and equilibrated with 25 mM Tris HCl pH 8.5 (anion ion exchange) or 10 mM MES-NaOH pH 5.5 (cation ion exchange) at the flow rates of 1, 5, or 10 mL/min.For anion ion exchange, 5 mg/mL BSA in the above buffer was then pumped onto the column until a break through in absorbance at 280 nm was seen.The flow was continued until a plateau in absorbance was achieved corresponding to 100% protein feed.Dynamic binding capacity was then calculated at 10% of the plateau value, correcting for any "dead volume" in the system and expressed as mg BSA/mL media volume.For cation ion exchange, 5 mg/mL lysozyme was used to test these resins in a similar manner to the anion ion exchange media. 
Part Number 描述 Pkg Price Qty
AcroSep 离子交换层析柱
20067-C001 DEAE Ceramic HyperD F, 1 mL, Orange 5/pkg Call
20066-C001 Q Ceramic HyperD F, 1 mL, Red 5/pkg Call
20062-C001 S Ceramic HyperD F, 1 mL, Blue 5/pkg Call
20050-C001 CM Ceramic HyperD F, 1 mL, Green 5/pkg Call
Ceramic HyperD® F Ion Exchange Chromatography Sorbents
20066-031 Q 陶瓷 HyperD F 25 毫升 Call
20066-023 Q 陶瓷 HyperD F 100 毫升 Call
20066-015 Q 陶瓷 HyperD F 1000 毫升 Call
20062-030 S Ceramic HyperD F 25 毫升 Call
20062-022 S Ceramic HyperD F 100 毫升 Call
20062-014 S Ceramic HyperD F 1000 毫升 Call
20067-039 DEAE Ceramic HyperD F 25 毫升 Call
20067-021 DEAE Ceramic HyperD F 100 毫升 Call
20067-013 DEAE Ceramic HyperD F 1000 毫升 Call
20050-035 CM Ceramic HyperD F 25 毫升 Call
20050-027 CM Ceramic HyperD F 100 毫升 Call
20050-019 CM Ceramic HyperD F 1000 毫升 Call
 

 


pall,颇尔,HyperD F 离子交换填料(Q、S、DEAE 和 CM)
上一条pall,颇尔,Centramate & Centramate PE 实验室中试切向流系统,FS001K10, FS002K10, FS005K10, FS007K10, FS012K10, FS010K10
下一条pall,颇尔,DMSO 专用 Acrodisc 针头过滤器,4433

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