pall,颇尔,HEA 和 PPA HyperCel 混合模式(疏水-离子交换)填料,20250-026, 20250-033, 20250-041, 20260-025, 20260-030, 20260-040

产品名称:
HEA 和 PPA HyperCel 混合模式(疏水-离子交换)填料
产品型号:
20250-026, 20250-033, 20250-041, 20260-025, 20260-030, 20260-040
产品描述:

产品介绍

pall,颇尔,HEA 和 PPA HyperCel 混合模式(疏水-离子交换)填料,20250-026, 20250-033, 20250-041, 20260-025, 20260-030, 20260-040
pall,颇尔,HEA 和 PPA HyperCel 混合模式(疏水-离子交换)填料

HEA 和 PPA HyperCel 混合模式(疏水-离子交换)填料

For Selectivities Not Accessible with
Traditional Ion Exchange or HIC

  • Chromatographic behavior “mixed-mode” based on a combination of electrostatic and hydrophobic properties of the protein and ligands
  • Provides direct hydrophobic capture of proteins at low ionic strength
  • Can be exploited to achieve discrimination of proteins having similar or very close isoelectric points, a separation that cannot be performed by methods like ion exchange
  • Can be used in a “physiological-like” environment (no need for pH adjustment of the feedstream; no, or limited, addition of salt)
  • Orthogonal to ion exchange or other chromatography steps

 

 
 
  • Fractionation of a protein mix
  • Separation of partially-purified polyclonal IgG from major plasma impurities
  • Process polishing steps
 
 

Average Particle Size

  • 90 微米

 

Bead Composition

  • High porosity cross-linked cellulose

 

Dynamic Binding Capacity for BSA (10% breakthrough)1

  • 40 - 60 mg/mL

 

配基

  • Aliphatic (HEA):己胺
  • Aromatic (PPA):苯丙胺

 

Adsorption pH

  • 7 - 9

 

Elution pH

  • By gradient or step elution, e.g. 7.0 - 2.6

 

Working pH

  • 2 - 12

 

Pressure Resistance

  • < 3 bar (300 kPa, 44 psi)

 

Storage Temperature

  • 2 - 8 °C (36 - 46 °F)

 

1Determined using 5 mg/mL BSA in PBS; flow rate:100 cm/h
 
 

Comparison Between HEA and PPA HyperCel, MEP HyperCel, Conventional HIC, and Anion Exchange Sorbents 

Comparison Between HEA and PPA HyperCel, MEP HyperCel, Conventional HIC, and Anion Exchange Sorbents

Adsorption/desorption of Bovine Serum Albumin (BSA), in PBS buffer, pH 7.4. Data shows that BSA is efficiently retained on both HEA and PPA HyperCel sorbents, but is poorly retained on MEP HyperCel sorbent (as this ligand is antibody-selective).In PBS, without lyotropic salt addition, low binding of BSA to both Phenyl and Hexyl HIC sorbents is observed.The anion exchange (DEAE) sorbent also did not bind BSA at these nonoptimal conditions (pH 7.4 and too high salt concentration).
 
AcroSep 混合模式层析柱(MEP、HEA、PPA、SDR)
Part Number 描述 Pkg Price Qty
HEA HyperCel Mixed-mode Chromatography Resin
20250-026 HEA HyperCel 25 毫升 Call
20250-033 HEA HyperCel 100 毫升 Call
20250-041 HEA HyperCel 1000 毫升 Call
PPA HyperCel Mixed-mode Chromatography Resin
20260-025 PPA HyperCel 25 毫升 Call
20260-030 PPA HyperCel 100 毫升 Call
20260-040 PPA HyperCel 1000 毫升 Call
 

 


pall,颇尔,HEA 和 PPA HyperCel 混合模式(疏水-离子交换)填料
上一条pall,颇尔,HA Ultrogel 羟基磷灰石填料,24775-082, 24775-025, 24775-017, 24775-041
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