Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples
- Ensures rapid processing of samples
- Typical recoveries are > 90%
- Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
- A wide range of MWCOs, color-coded for easy identification
- Constructed of low-binding polypropylene
- Ultrasonically welded seals prevent bypass or seal failure
- Fits standard centrifuge rotors that accept 1.5 mL tubes
- Concentrate, purify, and desalt oligonucleotides, DNA, RNA, and proteins
- Clean up labeling and PCR reactions
- Isolate DNA from agarose gel slices
- Separate oligonucleotides and RNA from acrylamide gels
- Concentrate PCR products regardless of size with 30K device if primer removal is required
材料成分
-
Nanosep 装置
Filter Media：Omega (modified polyethersulfone) ultrafiltration membrane
Sample Reservoir, Membrane Support Base, and Filtrate Receiver:聚丙烯 -
Nanosep MF Devices
Filter Media：Bio-Inert (modified nylon) and GHP (hydrophilic polypropylene) membranes
Sample Reservoir, Membrane Support Base, and Filtrate Receiver:聚丙烯
有效过滤面积
- 0.3 cm2
尺寸
- Overall Length (Fully Assembled with Cap):4.5 cm (1.8 in.)
Capacities
- Maximum Sample Volume:500 µL
- Final Concentrate Volume:15 µL
- Filtrate Receiver Volume:500 µL
- Hold-up Volume (Membrane/Support):< 5 微升
操作温度范围
- 0 - 40 °C (32 - 104 °F)
pH 范围
- Nanosep Devices: 1 - 14
- Nanosep MF Devices: 3 - 14
Maximum Centrifugal Force
- 14,000 x g
Centrifuge
- Fits rotors that accept 1.5 mL tubes
Sanitization
- Provided non-sterile.May be sanitized by filtering 70% ethanol through the device prior to use.
DNA Recovery as a Function of Device MWCO
A 500 μL sample of a 100 μg/mL DNA fragment solution containing 50 and 500 bp double-stranded DNA fragments was centrifuged at 5,000 x g in Nanosep devices to a final volume of 50 μL.Recovered samples were quantitated using absorbance at 260 nm.The 100K device was able to differentiate between the sizes of the DNA fragments.
Omega™ Membrane Has the Lowest Non-Specific DNA Binding
A 500 μL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device.The retained DNA was recovered in 40 μL TE (10 mM Tris, 1 mM EDTA, pH 8).The remaining radioactivity in the device was counted in a scintillation counter.A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample.Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries.In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule.Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g.Otherwise, DNA can be forced through many MWCO membranes regardless of size.
Centrifugal Device Spin Times
- 用于超滤的AcroPrep Advance 96 孔板
- AcroWell™ 96-well Membrane-bottom Plates with GHP Membrane
- AcroWell™ 96-well Membrane-bottom Plates with BioTrace™ PVDF and NT Membranes
- Acrodisc® Syringe Filters with GHP Membrane & GH Polypro (GHP) Membrane Disc Filters
- Acrodisc® Syringe Filters with Nylon Membrane & Nylaflo™ Membrane Disc Filters
- Acrodisc® Unit with Mustang™ E Membrane
- Acrodisc® Unit with Mustang™ Q Membrane
- Acrodisc® Unit with Mustang™ S Membrane
- BioTrace NT 纯硝化纤维素转印膜
- BioTrace PVDF 转印膜
- Biodyne® Transfer Membranes
- DMSO-Safe Acrodisc® Sterile Syringe Filter
- Ion Chromatography (IC) Acrodisc® Syringe Filters & Supor® (PES) Membrane Disc Filters
