pall,颇尔,Nanosep 和 Nanosep MF 浓缩离心管,OD003C33, OD003C34, OD003C35, OD010C33, OD010C34, OD010C35, OD030C33, OD030C34, OD030C35, OD100C33, OD100C34, OD100C35, OD300C33, OD300C34, OD300C35, ODM02C33, ODM02

产品名称:
Nanosep 和 Nanosep MF 浓缩离心管
产品型号:
OD003C33, OD003C34, OD003C35, OD010C33, OD010C34, OD010C35, OD030C33, OD030C34, OD030C35, OD100C33, OD100C34, OD100C35, OD300C33, OD300C34, OD300C35, ODM02C33, ODM02C34, ODM02C35, ODM45C33, ODM45C34, ODM45C35, ODGHPC34, ODGHPC35
产品描述:

产品介绍

pall,颇尔,Nanosep 和 Nanosep MF 浓缩离心管,OD003C33, OD003C34, OD003C35, OD010C33, OD010C34, OD010C35, OD030C33, OD030C34, OD030C35, OD100C33, OD100C34, OD100C35, OD300C33, OD300C34, OD300C35, ODM02C33, ODM02
pall,颇尔,Nanosep 和 Nanosep MF 浓缩离心管

Nanosep 和 Nanosep MF 浓缩离心管

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

  • Ensures rapid processing of samples 
  • Typical recoveries are > 90%
  • Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
  • A wide range of MWCOs, color-coded for easy identification
  • Constructed of low-binding polypropylene 
  • Ultrasonically welded seals prevent bypass or seal failure
  • Fits standard centrifuge rotors that accept 1.5 mL tubes
 
 
  • Concentrate, purify, and desalt oligonucleotides, DNA, RNA, and proteins
  • Clean up labeling and PCR reactions
  • Isolate DNA from agarose gel slices
  • Separate oligonucleotides and RNA from acrylamide gels
  • Concentrate PCR products regardless of size with 30K device if primer removal is required
 
 

材料成分

  • Nanosep 装置
      Filter Media:Omega (modified polyethersulfone) ultrafiltration membrane
      Sample Reservoir, Membrane Support Base, and Filtrate Receiver:聚丙烯
  • Nanosep MF Devices
      Filter Media:Bio-Inert (modified nylon) and GHP (hydrophilic polypropylene) membranes
      Sample Reservoir, Membrane Support Base, and Filtrate Receiver:聚丙烯

 

有效过滤面积

  • 0.3 cm2

 

尺寸

  • Overall Length (Fully Assembled with Cap):4.5 cm (1.8 in.)

 

Capacities

  • Maximum Sample Volume:500 µL
  • Final Concentrate Volume:15 µL
  • Filtrate Receiver Volume:500 µL
  • Hold-up Volume (Membrane/Support):< 5 微升

 

操作温度范围

  • 0 - 40 °C (32 - 104 °F)

 

pH 范围

  • Nanosep Devices: 1 - 14
  • Nanosep MF Devices: 3 - 14

 

Maximum Centrifugal Force

  • 14,000 x g

 

Centrifuge

  • Fits rotors that accept 1.5 mL tubes

 

Sanitization

  • Provided non-sterile.May be sanitized by filtering 70% ethanol through the device prior to use.
 
 

DNA Recovery as a Function of Device MWCO


Nanosep Centrifugal Device:DNA Recovery as a Function of Device MWCO
 
A 500 μL sample of a 100 μg/mL DNA fragment solution containing 50 and 500 bp double-stranded DNA fragments was centrifuged at 5,000 x g in Nanosep devices to a final volume of 50 μL.Recovered samples were quantitated using absorbance at 260 nm.The 100K device was able to differentiate between the sizes of the DNA fragments.

 

Omega™ Membrane Has the Lowest Non-Specific DNA Binding


Omega™ Membrane Has the Lowest Non-Specific DNA Binding

A 500 μL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device.The retained DNA was recovered in 40 μL TE (10 mM Tris, 1 mM EDTA, pH 8).The remaining radioactivity in the device was counted in a scintillation counter.A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample.Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries.In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule.Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g.Otherwise, DNA can be forced through many MWCO membranes regardless of size.

 

Centrifugal Device Spin Times


Nanosep Centrifugal Device Spin Times
Part Number 描述 Pkg Price Qty
Nanosep® Centrifugal Devices with Omega™ Membrane
OD003C33 3K, gray 24/pkg Call
OD003C34 3K, gray 100/pkg Call
OD003C35 3K, gray 500/pkg Call
OD010C33 10K, blue 24/pkg Call
OD010C34 10K, blue 100/pkg Call
OD010C35 10K, blue 500/pkg Call
OD030C33 30K, red 24/pkg Call
OD030C34 30K, red 100/pkg Call
OD030C35 30K, red 500/pkg Call
OD100C33 100K, clear 24/pkg Call
OD100C34 100K, clear 100/pkg Call
OD100C35 100K, clear 500/pkg Call
OD300C33 300K, orange 24/pkg Call
OD300C34 300K, orange 100/pkg Call
OD300C35 300K, orange 500/pkg Call
Nanosep MF Centrifugal Devices with Bio-Inert® Membrane
ODM02C33 0.2 µm, aqua 24/pkg Call
ODM02C34 0.2 µm, aqua 100/pkg Call
ODM02C35 0.2 µm, aqua 500/pkg Call
ODM45C33 0.45 µm, wildberry 24/pkg Call
ODM45C34 0.45 µm, wildberry 100/pkg Call
ODM45C35 0.45 µm, wildberry 500/pkg Call
采用 GHP 膜的 Nanosep MF 离心机
ODGHPC34 0.45 µm, clear 100/pkg Call
ODGHPC35 0.45 µm, clear 500/pkg Call
 

 


pall,颇尔,Nanosep 和 Nanosep MF 浓缩离心管
上一条pall,颇尔,Nanosep MF GHP 膜浓缩离心管,ODGHPC34, ODGHPC35
下一条pall,颇尔,Mustang XT Acrodisc 离子交换膜层析针头滤器,适合实验测试和工艺开发,MSTGXT25Q16, MSTGXT25S16

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