For the Purification of a Wide
Variety of Enzymes and Proteins
- Strong bond of dye-to-sorbent prevents leakage of the dye in normal working conditions
- Excellent chemical stability is attributed to the synthetic nature of Trisacryl sorbent and the enhanced stability of the ligand coupling mechanism
- Excellent mechanical stability allows high flow rates with working pressures up to 3 bar (300 kPa, 45 psi)
- Basic matrix is Trisacryl GF2000, a macroporous non-ionic sorbent.Cibacron♦ Blue F3GA dye is strongly bound to the matrix through a six-carbon spacer arm
- Albumin removal increases the resolution of both 1D and 2D electrophoresis
- Designed for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors
- The interaction mechanism between Cibacron Blue F3GA and proteins involves one or more of the following:stereospecific recognition of NAD analogs; electrostatic and hydrophobic interaction; and/or electron exchange
颗粒尺寸 | 40 - 80 微米 |
配基 | Cibacron blue 3GA |
排阻限制 | 107 Da |
Capacity for human albumin1 | ≥10 mg/mL |
Thermal stability | 2 to 121 °C |
Working pH | 4 至 10 |
清洁 pH | 1 to 12 (short term) |
Working pressure at 100 cm/hr | Up to 3 bar (45 psi) |