For High Throughput Chromatography Sorbent Screening and OptimizationAcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with 颇尔 chromatography sorbents, to enable early chromatography sorbent screening for protein purification.Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption.Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using 颇尔 1 mL or 5 mL PRC prepacked columns filled with the same sorbents.
The AcroPrep ScreenExpert plate packages offer:
- One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents
- Two optimization plates:one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent
- 50 μL of sorbent per well, allowing small sample consumption
- Manual or robotic workstation-assisted operations
- A disposable format in a convenient individual package
图 1Principle of Optimization of Purification Step (Post-Protein A Capture) of a Monoclonal Antibody Separation
* HCP = Host Cell Proteins
* MAb = Monoclonal Antibody
Types of Plates and Applications
|Part Number||Sorbents||配基||Separation Mode||应用|
|96WP3MIXM50||MEP, HEA and PPA HyperCel sorbents x 32 wells of each||
|混合模式||Screening of mixed-mode ligand selectivity and optimization|
MEP HyperCel 填料
x 96 wells
|4-mercapto-ethyl-pyridine||混合模式||Optimization of separation conditions on MEP HyperCel sorbent|
HyperCel STAR AX 填料
x 96 wells
|伯胺||Salt tolerant anion exchange||Optimization of separation by “salt-tolerant” anion exchange|
Plate Filled with MEP, HEA and PPA HyperCel Mixed-Mode Sorbents
MEP, HEA and PPA HyperCel mixed-mode sorbents are designed for protein capture and impurity removal from various biological feedstreams.They operate on the basis of a mixed-mode mechanism where both hydrophobic and ionic effects are predominant.They provide new selectivity, orthogonal to conventional chromatography steps like ion exchange, hydroxyapatite or other.
The AcroPrep ScreenExpert plate filled with these three sorbents is designed for selectivity screening to facilitate the choice of the most appropriate ligand for a specific separation.Detailed screening instructions are supplied with the plate.
Plate Filled with MEP HyperCel Mixed-Mode Sorbent
This plate is designed for optimization and fine tuning of separation conditions using 颇尔 MEP HyperCel mixed-mode sorbent, an efficient alternative to conventional hydrophobic interaction chromatography (HIC).Typical applications include post-protein A purification of monoclonal antibodies (host cell protein and aggregate removal) or capture or intermediate purification of various recombinant proteins.
Plate Filled with HyperCel STAR AX Anion Exchange Sorbent
HyperCel STAR AX sorbent is an anion exchanger based on a “salt tolerant” primary amine ligand.It brings new selectivity options for protein capture or contaminant removal, in a broad feedstock conductivity range from 2 to 15 mS/cm.This avoids dilution or ultrafiltration/diafiltration (UF/DF) of the sample, making process faster and providing economic benefits.
The corresponding AcroPrep ScreenExpert plate allows a fast way of screening and optimizing these new sorbents before transfer to PRC 1 mL and 5 mL prepacked columns.
|Plate size||128 x 86 x 33 mm|
|Filter media and type||0.45 μm Supor® hydrophilic polyethersulfone membrane (颇尔)|
|Part number for the empty plate||8129|
|Mode of operation||Vacuum using manifold or centrifuge|
|Volume of chromatography sorbent per well||50 μL in a total volume of 800 μL|
|Storage solution per well||750 μL of 1 M NaCl with 20% (v/v) ethanol|
|Working volume per well||100 to 500 μL|
|Maximum centrifugation force||700 x g|
|Maximum vacuum||0.5 bar g (375 mm Hg)|
Transfer to Column Chromatography on PRC Prepacked Columns
All conditions selected on plates should be confirmed by column chromatography prior to scale up.1 mL or 5 mL PRC prepacked columns filled with the relevant sorbents allow convenient verification and small preparative work.Columns allow the estimation of chromatography parameters such as dynamic binding capacity for proteins.For scale-up to small preparative work, two 5 mL PRC columns can be connected in series to increase the bed height to 20 cm and model real conditions in pilot scale.Refer to 颇尔 Application Note USD 2718 for examples.
|AcroPrep ScreenExpert Plates|
|MEP, HEA and PPA HyperCel Mixed-Mode Sorbents||96WP3MIXM50|
|MEP HyperCel Mixed-Mode Sorbent||96WPMEP50|
|HyperCel STAR AX Anion Exchange Sorbent||96WPSTARAX50|
|AcroPrep Advance 96-Well Filter Plate||8129|
|PRC Prepacked Columns:|
|MEP HyperCel PRC Column 5 x 50, 1 mL||PRC05X050MEPHCEL01|
|MEP HyperCel PRC Column 8 x 100, 5 mL||PRC08X100MEPHCEL01|
|HEA HyperCel PRC Column 5 x 50, 1 mL||PRC05X050HEAHCEL01|
|HEA HyperCel PRC Column 8 x 100, 5 mL||PRC08X100HEAHCEL01|
|PPA HyperCel PRC Column 5 x 50, 1 mL||PRC05X050PPAHCEL01|
|PPA HyperCel PRC Column 8 x 100, 5 mL||PRC08X100PPAHCEL01|
|HyperCel STAR AX PRC Column 5 x 50, 1 mL||PRCSTARAX1ML|
|HyperCel STAR AX PRC Column 8 x 100, 5 mL||PRCSTARAX5ML|
|MEP HyperCel 200 μL, row of 8||SR2MEP|
|HEA HyperCel 200 μL, row of 8||SR2HEA|
|PPA HyperCel 200 μL, row of 8||SR2PPA|
|HyperCel STAR AX 200 μL, row of 8||SR2STARAX|
|MEP HyperCel 600 μL, row of 8||SR6MEP|
|HEA HyperCel 600 μL, row of 8||SR6HEA|
|PPA HyperCel 600 μL, row of 8||SR6PPA|
|HyperCel STAR AX 600 μL, row of 8||SR6STARAX|
|96-well RoboColumn array plate||SR96WAP|
For other types of plates, please contact us.