
Convenient and Reliable Concentration, Purification, and Diafiltration of 20 to 60 mL Biological Samples
- Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes
- Provides high recoveries, typically > 90%
- Low protein-binding Omega™ membrane and polysulfone housing minimize losses due to non-specific binding
- Versatile Omega membrane is available in a variety of MWCOs, color-coded for easy identification
- Built-in deadstop prevents spinning to dryness
- Unique sealing mechanism prevents retentate leakage and filtrate contamination
- Economical - Sample reservoir and filtrate receiver can be sanitized or autoclaved, and reused
- Concentrating and desalting proteins
- Exchanging buffer or removing salt from chromatography eluates and gradient fractions
- Separating biomolecules from cell culture supernatants
- Concentrating or removing viruses
- Performing crude fractionation of protein mixtures
- Removing debris and particulates from cell lysates
材料成分
- Filter Media:Omega (modified polyethersulfone) membrane
- Sample Reservoir and Filtrate Receiver:聚砜
- Sample Reservoir Cap:聚乙烯
- Insert Without Membrane:High density polyethylene
- Filtrate Receiver Cap and Insert Release:聚丙烯
有效过滤面积
- 15.2 cm2
尺寸
- Outside Diameter (Maximum):6 cm (2.4 in.)
- Overall Height (Fully Assembled with Cap):11.3 cm (4.5 in.)
Capacities
- Maximum Sample Volume:60 毫升
- Final Concentrate Volume:3.5 - 4 毫升
- Maximum Filtrate Receiver Volume:60 毫升
- Hold-up Volume (membrane/support):0.2 毫升
操作温度范围
- 0 - 40 °C (32 - 104 °F)
pH 范围
- 1 - 14
Centrifuge
- Swinging bucket rotor is required that accepts flat-bottomed 250 mL bottles and is capable of spinning at up to 3,000 x g
Sanitization
- Provided non-sterile.The entire device, including the filter media, may be sanitized by filtering 70% ethanol through it prior to use.The sample reservoir and the filtrate receiver can be autoclaved.Do not autoclave the filter media.

1 mg/mL BSA solution was processed in each of the above devices until a 15-fold concentration is achieved.
Concentration Time

Concentrate dilute protein samples in less than 30 minutes with 10, 30, and 100K Jumbosep devices.