Quickly Concentrates Up To
20 mL of Biological Sample
- Rapidly concentrates 20 mL sample volumes to 0.5 mL
- Provides high recoveries, typically > 90%
- Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding
- Versatile Omega membrane is available in a variety of MWCOs
- Built-in deadstop prevents spinning to dryness
- Color-coded for easy identification
超滤
- Concentrate and desalt proteins
- Exchange buffer or remove salt of chromatography eluates and gradient fractions
- Recover proteins or other molecules from cell culture supernatants
微滤
- Remove particulate from aqueous solutions and clinical samples
材料成分
- Filter Media：Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
- Sample Reservoir, Filtrate Receiver, and Cap:聚丙烯
- Paddle:聚乙烯
有效过滤面积
- 7.2 cm2
尺寸
- 直径：29 mm (1.2 in.)
- Length:12.0 cm (4.7 in.)
操作温度范围
- 0 - 40 °C (32 - 104 °F)
Capacities
- Maximum Sample Volume:20 毫升
-
Final Concentrate Volume:
450 μL (swinging bucket)
1.2 - 1.5 mL (45º angle rotor)
1.5 mL (34º angle rotor) - Filtrate Receiver Volume:22 毫升
- Hold-Up Volume:80 μL (membrane and paddle)
pH 范围
- 1 - 14
Maximum Centrifugal Force
- 5,000 x g (ultrafiltration)
- 14,000 x g (microfiltration)
Centrifuge
- Fits centrifuges that accept standard 50 mL conical end tubes
Sanitization
- Provided non-sterile.May be sanitized by filtering 70% ethanol through thedevice prior to use.
Macrosep Advance Centrifugal Devices:Reduced Spin Time
Protein solutions were processed in each of the Macrosep Advance devices.Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g.Solutions are 3K:Protamine Sulfate, 0.1% in 1X PBS; 10K:Cytochrome C, 0.025% in 1X PBS; 30K:IgG, 0.1% in 1X PBS; and 100K:Apoferritin, 0.1% in 1X PBS.
Macrosep Advance Centrifugal Devices:截留率
Protein solutions were processed in each of the Macrosep Advance devices.Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO.Solutions were 3K:Protamine Sulfate, 0.1% 1X PBS; 10K:Cytochrome C, 0.025% in 1X PBS; 30K:IgG, 0.1% in 1X PBS; and 100K:Apoferritin, 0.1% in 1X PBS.
