pall,颇尔,MEP HyperCel 混合模式填料,12035-010, 12035-028, 12035-036

产品名称:
MEP HyperCel 混合模式填料
产品型号:
12035-010, 12035-028, 12035-036
产品描述:

产品介绍

pall,颇尔,MEP HyperCel 混合模式填料,12035-010, 12035-028, 12035-036
pall,颇尔,MEP HyperCel 混合模式填料

MEP HyperCel 混合模式填料

For the Capture and Purification of
Monoclonal and Polyclonal Antibodies

  • High purity in a single step - Product purities of 70% to 90% or greater are typically achieved
  • Rapid sample processing - Dynamic binding capacities of > 30 mg IgG per mL of sorbent (at 10% breakthrough) are routinely achieved
  • Exhibits high IgG capacity, independent of sub-class or species
  • Allows direct sample loading without adjustment of pH or ionic strength
  • Effective capture, even with feedstocks as dilute as 50 to 100 μg IgG/mL
  • Offers easy cleaning with sodium hydroxide

 

 
 
  • Capture and purification of antibodies from a broad range of sources such as animal sera, ascites fluid, and cell culture supernatant.
  • Independent of subclass or species.Weakly-binding variants (e.g., murine IgG, rat IgG) are well retained.
  • Alternative to traditional HIC sorbents for capture of hydrophobic molecules.
  • Has been studied in a broad range of applications including isolation of antibodies from sweet-whey and colostrum; isolation of antibodies from transgenic plant and animal sources; and, isolation of IgA and selected fusion proteins
 
 

颗粒尺寸

  • 80 - 100 µm (average)

 

Bead Composition

  • High porosity cross-linked cellulose

 

Dynamic Binding Capacity for Human IgG (10% breakthrough)1

  • > 20 毫克/毫升

 

配基

  • 4-Mercaptoethylpyridine (4-MEP)

 

Ligand Density

  • 80 - 125 µmol/mL

 

Working pH

  • 2 - 12

 

Pressure Resistance

  • < 3 bar (300 kPa, 44 psi)

 

Storage Temperature

  • 2 - 8 °C (36 - 46 °F)

 

1Determined using 5 mg/mL human IgG in PBS, flow rate 60 cm/h.
 
 

Monoclonal Antibodies Purification on MEP
HyperCel Resin from Cell Culture Supernatants

Panel A:Serum-free Cell Culture Media 

Monoclonal Antibodies Purification on MEP HyperCel Resin from Cell Culture Supernatants

Panel B:5% Serum-supplemented Cell Culture Media

Monoclonal Antibodies Purification on MEP HyperCel Resin from Cell Culture Supernatants

Sample A = 300 mL protein-free cell culture supernatant.Sample B = 300 mL cell culture supernatant containing 5% fetal bovine serum; Equilibration:50 mM Tris-HCI, pH 8; Elution:50 mM acetate, pH4; Flow rate:70 cm/h.In curve B, (a) and (b) are respectively water and 25 mM sodium caprylate washings; SDS-PAGE (reduced conditions):(1) = crude sample; (2) = purified IgG; (H) = heavy chain; (L) = light chain.
 
AcroSep 混合模式层析柱(MEP、HEA、PPA、SDR)
Part Number 描述 Pkg Price Qty
MEP HyperCel 疏水性电荷诱导层析 (HCIC) 树脂
12035-010 MEP HyperCel 25 毫升 Call
12035-028 MEP HyperCel 100 毫升 Call
12035-036 MEP HyperCel 1000 毫升 Call
 

 


pall,颇尔,MEP HyperCel 混合模式填料
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