For Ion Exchange and Mixed-Mode Chromatography Laboratory Applications
- Convenient – Ready-to-use 1 and 5 mL pre-packed columns.
- Easy to use – Direct connection to commonly-used laboratory chromatography systems, such as ÄKTA♦ systems.
- Consistent – Screen 颇尔 ion exchange and mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.
- Versatile – Two or more columns can be connected in series to increase the column height and more closely model real pilot scale conditions or scale-down applications.
离子交换层析
Q and S HyperCel™ sorbents are composed of a rigid cellulose matrix that has excellent flow properties and generates low back pressure.These sorbents provide:- High dynamic binding capacity at short residence time.
- Either capture or intermediate step use.
- Fast re-equilibration allowing buffer and time savings.
- Direct scale-up to pilot or production scale columns.
- High dynamic binding capacity (DBC) at short residence time (2 minutes or lower)
- Direct capture of protein from undiluted feedstock at moderate or high conductivity
- Excellent flow rate properties for fast feedstock processing
- Distinctive selectivity, consistent in a broad conductivity range (2-15 mS/cm)
- Enhanced process economics
- Good dynamic binding capacity independent of flow rate.
- Direct capture with high binding capacity for IgG at conductivities of 10-15 mS/cm on CM Ceramic HyperD sorbent.
应用
- Native and recombinant proteins
- Plasmids
- 疫苗
- Monoclonal and polyclonal antibodies
- 血液制品
- 生物制药
混合式层析
MEP, HEA, and PPA HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants.They provide a unique separation mechanism different from conventional methods.All ligands operate predominantly by hydrophobic interaction.This "HIC-like" interaction typically takes place without the addition of lyotropic salt.应用
-
MEP HyperCel 填料
- Antibody capture
- Alternative to conventional hydrophobic interaction
-
MEP, HEA, and PPA HyperCel sorbents
- No salt/low salt alternative to hydrophobic interaction
材料成分
Body and End Caps:Molded polypropylene17 μm Frit:PP/PE (polypropylene/polyethylene)
Outer Dimensions
1 mL:10 x 100 mm5 mL:11.5 x 140 mm
Connections
Built-in 10-32 fittingsFor HPLC/MPLC/ÄKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings.For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.
最大操作压力
1 mL:20 barg (290 psig)5 mL:30 barg (435 psig)
Volume
1 mL:5 mm ID x 50 mm5 mL:8 mm ID x 100 mm
Storage Solution
HyperCel STAR AX and Q/S HyperCel Sorbents:30% isopropanol/100 mM sodium phosphate, pH 4.3Ceramic HyperD F Sorbent:20% ethanol/150 mM NaCl
Working Pressure
HyperCel STAR AX and Q/S HyperCel Sorbents:< 0.5 barg (7 psig)Ceramic HyperD F Sorbent:< 1.5 barg (22 psig)
Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
| 离子交换 | Chemistry | 平均颗粒尺寸（微米） | Ionizable Groups (μEq/mL) | Dynamic Capacity (mg/mL)1 |
| Q HyperCel |
Quaternary amine |
75 | 99-138 | ≥ 1602 |
| S HyperCel | 磺酸 | 75 | 59-84 | ≥ 1353 |
| Q 陶瓷 HyperD F |
Quaternary amine |
50 | ≥ 250 | ≥ 854 |
| CM Ceramic HyperD F | 羧 | 50 | ≥ 250 | ≥ 605 |
| HyperCel STAR AX | – | 80 微米 | – | >100 mg8 8 5 mg/mL BSA in 25 mM Tris-HCl, 0.14 M NaCl at 2 min residence time. |
| 混合模式 | 配基 | 平均颗粒尺寸（微米） | Ligand Density (μmole/mL) | Dynamic Capacity (mg/mL)1 |
| MEP HyperCel | 4-mercaptoethyl- pyridine (pKa = 4.8) | 90 | 80-125 | ≥ 206 |
| HEA HyperCel | Hexylamine (aliphatic) (pKa = 8.0) | 90 | 58-84 | ≥ 407 |
| PPA HyperCel | Phenylpropyla mine (aromatic) (pKa = 8.0) | 90 | 58-80 | ≥ 407 |
1 Determined at 10% breakthrough using:
2 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.5 at 2 minute residence time.
3 5 mg/mL human IgG in 50 mM sodium acetate, pH 4.5 at 2 minute residence time.
4 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h.
5 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h.
6 5 mg/mL human IgG in PBS, flow rate 60 cm/h.
7 5 mg/mL BSA in PBS, flow rate 100 cm/h.
图 1
Separation of Cytochrome C, BSA, and Human Transferrin on a PRC Q Ceramic HyperD F Pre-Packed Column
Column:Q Ceramic HyperD F PRC pre-packed column (5 mm ID x 50 mm).体积：1 mL linear flow rate:150 cm/h.Equilibration and wash:50 mM Tris-HCl, pH 8.6. Load:100 μL (5 mg/mL cytochrome C, 20 mg/mL BSA, and 20 mg/mL human transferrin in equilibration buffer).Elution:50 mM
图 2
Separation of Model Proteins (Ovalbumin, ß-lactoglobulin, Cytochrome C, and Lysozyme) on 1 mL PRC S HyperCel Pre- Packed Columns and Scale Up on 5 mL Column, Constant Residence Time of 2 Minutes
Columns:S HyperCel PRC pre-packed columns of 1 mL (5 mm ID x 50 mm) and 5 mL (8 mm ID x 100 mm).Load:for 1 mL column – 100 μL ovalbumin (10 mg/mL), ß-lactoglobulin (10 mg/mL), cytochrome C (2.5 mg/mL), and lysozyme (5 mg/mL).For 5 mL column – 500 μL of same proteins.Equilibration:50 mM Na acetate, pH 4.5. Elution:50 mM Na acetate, pH 4.5 + 0.5 M NaCl by linear gradient from 0 to 100%.
图 3
Separation of Lysozyme, α-Chymotrypsinogen, Ovalbumin, and BSA on a PRC PPA HyperCel Pre-Packed Column
Column:PPA HyperCel PRC pre-packed column (5 mm ID x 50 mm).体积：1 mL linear flow rate:75 cm/h.Equilibration and wash:PBS, pH 7.4. Load:500 μL (lysozyme, α-chymotrypsinogen, ovalbumin, and BSA, all at 2 mg/mL in equilibration buffer).Elution:by pH step gradient in phosphate/citrate buffer, pH 7.0, 5.4, and 2.6.
- AcroSep™ Chromatography Columns – Pre-packed 1 mL chromatography columns for accelerated protein purification and analysis.
- LRC Chromatography Columns – Empty glass columns for preparative and process optimization applications.
- Acrodisc® Units with Mustang® Membrane – Disposable chromatography units with high binding capacities and fast flow rates.
