pall,颇尔,PRC 层析预装柱,PRC05X050QHCEL01, PRC05X050SHCEL01, PRC05X050QCHDF01, PRC05X050CMCHDF01, PRC08X100QHCEL01, PRC08X100SHCEL01, PRCSTARAX1ML, PRCSTARAX5ML, PRC05X050MEPHCEL01, PRC05X050HEAHCEL01, PRC05

产品名称:
PRC 层析预装柱
产品型号:
PRC05X050QHCEL01, PRC05X050SHCEL01, PRC05X050QCHDF01, PRC05X050CMCHDF01, PRC08X100QHCEL01, PRC08X100SHCEL01, PRCSTARAX1ML, PRCSTARAX5ML, PRC05X050MEPHCEL01, PRC05X050HEAHCEL01, PRC05X050PPAHCEL01, PRC08X100MEPHCEL01, PRC08X100HEAHCEL01, PRC08X100PPAH
产品描述:

产品介绍

pall,颇尔,PRC 层析预装柱,PRC05X050QHCEL01, PRC05X050SHCEL01, PRC05X050QCHDF01, PRC05X050CMCHDF01, PRC08X100QHCEL01, PRC08X100SHCEL01, PRCSTARAX1ML, PRCSTARAX5ML, PRC05X050MEPHCEL01, PRC05X050HEAHCEL01, PRC05
pall,颇尔,PRC 层析预装柱

PRC 层析预装柱

For Ion Exchange and Mixed-Mode Chromatography Laboratory Applications

  • Convenient – Ready-to-use 1 and 5 mL pre-packed columns.
  • Easy to use – Direct connection to commonly-used laboratory chromatography systems, such as ÄKTA systems.
  • Consistent – Screen 颇尔 ion exchange and mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.
  • Versatile – Two or more columns can be connected in series to increase the column height and more closely model real pilot scale conditions or scale-down applications.
 

离子交换层析

Q and S HyperCel™ sorbents are composed of a rigid cellulose matrix that has excellent flow properties and generates low back pressure.These sorbents provide:
  • High dynamic binding capacity at short residence time.
  • Either capture or intermediate step use.
  • Fast re-equilibration allowing buffer and time savings.
  • Direct scale-up to pilot or production scale columns.
HyperCel STAR AX sorbent is an industry-scalable anion exchange chromatography sorbent designed for high productivity protein capture at moderate to physiological or higher salt conductivity (2 to 15 mS/cm), typical of undiluted biological feedstocks (i.e., mammalian cell culture supernatants, E. coli feedstock, plasma, others…).
  • High dynamic binding capacity (DBC) at short residence time (2 minutes or lower)
  • Direct capture of protein from undiluted feedstock at moderate or high conductivity
  • Excellent flow rate properties for fast feedstock processing
  • Distinctive selectivity, consistent in a broad conductivity range (2-15 mS/cm)
  • Enhanced process economics
Q and CM Ceramic HyperD® sorbents have a high capacity hydrogel polymerized within the gigapores of a rigid ceramic bead.Typical features include:
  • Good dynamic binding capacity independent of flow rate.
  • Direct capture with high binding capacity for IgG at conductivities of 10-15 mS/cm on CM Ceramic HyperD sorbent.
Ceramic HyperD and HyperCel sorbents have different selectivities.The PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

应用

  • Native and recombinant proteins
  • Plasmids
  • 疫苗
  • Monoclonal and polyclonal antibodies
  • 血液制品
  • 生物制药

混合式层析

MEP, HEA, and PPA HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants.They provide a unique separation mechanism different from conventional methods.All ligands operate predominantly by hydrophobic interaction.This "HIC-like" interaction typically takes place without the addition of lyotropic salt.

应用

  • MEP HyperCel 填料
    • Antibody capture
    • Alternative to conventional hydrophobic interaction
  • MEP, HEA, and PPA HyperCel sorbents
    • No salt/low salt alternative to hydrophobic interaction
 

材料成分

Body and End Caps:Molded polypropylene
17 μm Frit:PP/PE (polypropylene/polyethylene)

Outer Dimensions

1 mL:10 x 100 mm
5 mL:11.5 x 140 mm

Connections

Built-in 10-32 fittings
For HPLC/MPLC/ÄKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings.For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

最大操作压力

1 mL:20 barg (290 psig)
5 mL:30 barg (435 psig)

Volume

1 mL:5 mm ID x 50 mm
5 mL:8 mm ID x 100 mm

Storage Solution

HyperCel STAR AX and Q/S HyperCel Sorbents:30% isopropanol/100 mM sodium phosphate, pH 4.3
Ceramic HyperD F Sorbent:20% ethanol/150 mM NaCl

Working Pressure

HyperCel STAR AX and Q/S HyperCel Sorbents:< 0.5 barg (7 psig)
Ceramic HyperD F Sorbent:< 1.5 barg (22 psig)
Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.

 
离子交换 Chemistry 平均颗粒尺寸(微米) Ionizable Groups (μEq/mL) Dynamic Capacity (mg/mL)1
Q HyperCel Quaternary
amine
75 99-138 ≥ 1602
S HyperCel 磺酸 75 59-84 ≥ 1353
Q 陶瓷 HyperD F Quaternary
amine
50 ≥ 250 ≥ 854
CM Ceramic HyperD F 50 ≥ 250 ≥ 605
HyperCel STAR AX 80 微米 >100 mg8 8 5 mg/mL BSA in 25 mM Tris-HCl, 0.14 M NaCl at 2 min residence time.
 

 
混合模式 配基 平均颗粒尺寸(微米) Ligand Density (μmole/mL) Dynamic Capacity (mg/mL)1
MEP HyperCel 4-mercaptoethyl- pyridine (pKa = 4.8) 90 80-125 ≥ 206
HEA HyperCel Hexylamine (aliphatic) (pKa = 8.0) 90 58-84 ≥ 407
PPA HyperCel Phenylpropyla mine (aromatic) (pKa = 8.0) 90 58-80 ≥ 407
 
1 Determined at 10% breakthrough using:
2 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.5 at 2 minute residence time.
3 5 mg/mL human IgG in 50 mM sodium acetate, pH 4.5 at 2 minute residence time.
4 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h.
5 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h.
6 5 mg/mL human IgG in PBS, flow rate 60 cm/h.
7 5 mg/mL BSA in PBS, flow rate 100 cm/h.
 

图 1

Separation of Cytochrome C, BSA, and Human Transferrin on a PRC Q Ceramic HyperD F Pre-Packed Column
图 1
Column:Q Ceramic HyperD F PRC pre-packed column (5 mm ID x 50 mm).体积:1 mL linear flow rate:150 cm/h.Equilibration and wash:50 mM Tris-HCl, pH 8.6. Load:100 μL (5 mg/mL cytochrome C, 20 mg/mL BSA, and 20 mg/mL human transferrin in equilibration buffer).Elution:50 mM


图 2

Separation of Model Proteins (Ovalbumin, ß-lactoglobulin, Cytochrome C, and Lysozyme) on 1 mL PRC S HyperCel Pre- Packed Columns and Scale Up on 5 mL Column, Constant Residence Time of 2 Minutes
图 2

图 2
Columns:S HyperCel PRC pre-packed columns of 1 mL (5 mm ID x 50 mm) and 5 mL (8 mm ID x 100 mm).Load:for 1 mL column – 100 μL ovalbumin (10 mg/mL), ß-lactoglobulin (10 mg/mL), cytochrome C (2.5 mg/mL), and lysozyme (5 mg/mL).For 5 mL column – 500 μL of same proteins.Equilibration:50 mM Na acetate, pH 4.5. Elution:50 mM Na acetate, pH 4.5 + 0.5 M NaCl by linear gradient from 0 to 100%.


图 3

Separation of Lysozyme, α-Chymotrypsinogen, Ovalbumin, and BSA on a PRC PPA HyperCel Pre-Packed Column
图 3
Column:PPA HyperCel PRC pre-packed column (5 mm ID x 50 mm).体积:1 mL linear flow rate:75 cm/h.Equilibration and wash:PBS, pH 7.4. Load:500 μL (lysozyme, α-chymotrypsinogen, ovalbumin, and BSA, all at 2 mg/mL in equilibration buffer).Elution:by pH step gradient in phosphate/citrate buffer, pH 7.0, 5.4, and 2.6.
  • AcroSep™ Chromatography Columns – Pre-packed 1 mL chromatography columns for accelerated protein purification and analysis.
  • LRC Chromatography Columns – Empty glass columns for preparative and process optimization applications.
  • Acrodisc® Units with Mustang® Membrane – Disposable chromatography units with high binding capacities and fast flow rates.
Part Number 描述 Pkg Price Qty
PRC Pre-Packed Chromatography Columns with Ion Exchange Sorbents
PRC05X050QHCEL01 Q HyperCel sorbent, 5 x 50 1 毫升 Call
PRC05X050SHCEL01 S HyperCel sorbent, 5 x 50 1 毫升 Call
PRC05X050QCHDF01 Q Ceramic HyperD F sorbent, 5 x 50 1 毫升 Call
PRC05X050CMCHDF01 CM Ceramic HyperD F sorbent, 5 x 50 1 毫升 Call
PRC08X100QHCEL01 Q HyperCel sorbent, 8 x 100 5 毫升 Call
PRC08X100SHCEL01 S HyperCel sorbent, 8 x 100 5 毫升 Call
PRCSTARAX1ML HyperCel STAR AX sorbent 5 x 50 1 毫升 Call
PRCSTARAX5ML HyperCel STAR AX sorbent 8 x 100 5 毫升 Call
PRC Pre-Packed Chromatography Columns with Mixed-Mode Sorbents
PRC05X050MEPHCEL01 MEP HyperCel sorbent, 5 x 50 1 毫升 Call
PRC05X050HEAHCEL01 HEA HyperCel sorbent, 5 x 50 1 毫升 Call
PRC05X050PPAHCEL01 PPA HyperCel sorbent, 5 x 50 1 毫升 Call
PRC08X100MEPHCEL01 MEP HyperCel sorbent, 8 x 100 5 毫升 Call
PRC08X100HEAHCEL01 HEA HyperCel sorbent, 8 x 100 5 毫升 Call
PRC08X100PPAHCEL01 PPA HyperCel sorbent, 8 x 100 5 毫升 Call
 

 


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