For the Purification of Biological
Molecules that Bind to Heparin
- Design combines the desirable characteristics of a soft, high speed, high capacity hydrogel with the high dimensional stability of a rigid bead
- Leakage is minimized due to the stable chemical link of the heparin molecule to the sorbent
- Rapid packing due to the high density of the bead
- Can be packed in a variety of column sizes
- Enzymes (lipoprotein lipase, coagulation enzymes, superoxide dismutase)
- Growth factors (fibroblast growth factor, Schwan cell growth factor)
- Extracellular matrix proteins (fibronectin, vitronectin)
- Nucleic acid binding protein, hormone receptors, and lipoproteins
Average Particle Size
- 80 微米
Dynamic Binding Capacity for Human ATIII (10% Breakthrough)1
- > 25 mg/mL
Immobilized Heparin/mL Sorbent
- 5 - 10 mg/mL
- 3 - 13
- 70 bar (7,000 kPa, 1,000 psi)
- 2 - 8 ° C (36 - 46 ° F)
1Determined using human ATIII at 72.5 UI/mL in 20 mM TrisHCl, 0.3 M NaCl, pH 7.4. Elution with 20 mM Tris HCl, 2 M NaCl, pH 7.4 at a flow rate of 600 cm/h, 10 cm (4 in.) bed height.
Dynamic Binding Capacity vs.Linear Velocity
Column dimensions:0.46 cm I.D. x 10 cm; Sample:HU ATIII at 72.5 μL/mL; Equilibration buffer:20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.
Pressure vs.Linear Flow Velocity
Column dimensions:0.46 cm I.D. x 10 cm; Buffer:20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.