pall,颇尔,用于 DNA 提纯的 AcroPrep Advance 孔板,8032, 8132

pall,颇尔,用于 DNA 提纯的 AcroPrep Advance 孔板

Maximum Yields of High Quality DNA

• Optimized for maximum binding yield of DNA from a variety of sample types
• New well geometry results in reduced hold-up volume and higher recovery of DNA
• New outlet tip geometry provides direct flow of samples into receiver plate without concerns of crosscontamination
• Enhanced membrane ensures high yields of contaminant-free DNA for downstream applications
• Manufactured in accordance with SBS guidelines, allowing entire DNA purification process to be performed on automated equipment

• Plasmid DNA purification
• Genomic DNA purification

材料成分

• Filter Media：DNA binding (borosilicate glass without binder) media
• Plate Housing:Polyproylene
• Lid:Polystyrene

 

尺寸

• Length:12.8 cm (5.0 in.)
• Width:8.6 cm (3.4 in.)
• Height With Lid:1.8 cm (0.7 in.) (350 μL only)
• Height Without Lid:350 μL, 1.4 cm (0.6 in.);1 mL:3.3 cm (1.3 in.)

 

Well-Bottom Area

• 0.25 cm2

 

Recommended Working Volume

• 350 μL: < 300 μL
• 1 mL: < 900 μL

 

Recommended Operating Vacuum

• > 25.4 cm Hg (10 in.Hg)

Recommended Centrifugal Force

• 1,500 x g

 

Typical Processing Time

• Vacuum:2 seconds
• Centrifuge:< 2 minutes

 

一般滞留量

• Optimized for maximum binding Vacuum:19 μL

Plasmid DNA Yield Superior to Competitive Plates

pCAT plasmid DNA yield (OD260) using indicated DNA purification plates starting with 1 mL (颇尔 and Competitor B) or 1.5 mL (Competitor A) overnight culture DH5α.Purification using plate manufacturer’s recommended protocol using 350 μL plates.Error bars indicate standard error (n > 6).

 

Quality DNA Consistently Purified

	Elution Volume (µL)	Recovered Volume (µL)	OD260/280	Concentration (ng/µL)	Total Yield (µg/well)
颇尔	70	55-65	1.95 ± 0.01	131	7.7
Competitor A	50	30	1.98 ± 0.01	155	4.6
Competitor B	70	45-60	2.04 ± 0.05	108	5.6

OD_260/280 of pooled purified pDNA (n > 6).High quality DNA has an OD260/280 value between 1.7-2.0.

 

Related Literature

• 海报：针对不同存储条件下的样品，使用 96 孔 DNA 结合板和液体处理机器人从全血中离析 DNA 的稳定性和再现性
• 应用说明：高效多孔滤板蛋白纯化方案
• 应用说明：原核培养中的改造质粒 DNA 的纯化

 

相关产品

• AcroPrep Advance Filter Plates for Multiplexing ensure assay reproducibility. 
• AcroPrep Advance Filter Plates for Neonatal Screening for consitent performance and accurate results.
• AcroPrep Advance Filter Plates for Protein Purification have a versatile format for multiple purification strategies.
• AcroPrep Advance Filter Plates for Lysate Clearance provide efficient clarification of highly particulated samples.
• AcroPrep Advance Filter Plates for Ultrafiltration typically result in > 90% recovery of target biomolecules.
• AcroPrep Advance Filter Plates for Solvent Filtration provide compatibility with harsh organics.
• AcroPrep Advance Filter Plates for Aqueous Filtration for fast processing with efficient removal of particulates.
• AcroPrep 384-well Filter Plates for superior performance in high throughput sample preparation applications.
• Centrifugal Devices concentrate and purify samples of < 50 μL to 60 mL with efficient recovery and low non-specific binding.
• Chromatography Resins simplify protein purification and fractionation for biomarker discovery, proteomics research and drug development.
• Vacuum Manifold fits AcroWell and AcroPrep filter plates tightly for vacuum filtration.

Part Number	描述	Pkg	Price	Qty
用于DNA提纯的 AcroPrep Advance 滤板
8032	350 μL, DNA binding	10/pkg	Call
8132	1 mL, DNA binding	5/pkg	Call
配件及替换部件
5017	多孔板真空歧管	1/pkg	Call
5225	Adapter collar for centrifugation	2/pkg	Call
5226	Adapter for PCR receiver plate	2/pkg	Call
5230	Cap mat for incubation	5/pkg	Call
8001	AcroPrep Advance multi-well plate lids	10/pkg	Call

pall,颇尔,用于 DNA 提纯的 AcroPrep Advance 孔板