pall,颇尔,SoloHill Microcarriers - Animal Protein Coated,C-215-020, C-215-030, C-215-040, C-215-050, C-215-060, C-215-070, C-215-080, C-221-020, C-221-030, C-221-040, C-221-050, C-221-060, C-221-070, C-

产品名称:
SoloHill Microcarriers - Animal Protein Coated
产品型号:
C-215-020, C-215-030, C-215-040, C-215-050, C-215-060, C-215-070, C-215-080, C-221-020, C-221-030, C-221-040, C-221-050, C-221-060, C-221-070, C-221-080, C-421-020, C-421-030, C-421-040, C-421-050, C-421-060, C-421-070, C-421-080, F-215-020, F-215-03
产品描述:

产品介绍

pall,颇尔,SoloHill Microcarriers - Animal Protein Coated,C-215-020, C-215-030, C-215-040, C-215-050, C-215-060, C-215-070, C-215-080, C-221-020, C-221-030, C-221-040, C-221-050, C-221-060, C-221-070, C-
pall,颇尔,SoloHill Microcarriers - Animal Protein Coated

SoloHill® Microcarriers - Animal Protein Coated

Animal Protein Coated Microcarriers

Anchorage dependent cell lines rely heavily on optimal substrate conditions.Collagen has been shown to be an effective promoter of cell attachment and growth in monolayer cultures.With collagen coated microcarriers, processes can be transferred directly out of collagen based monolayers and moved into space efficient 3D cultures with minimal development time.This upgrade allows improved process control and process economics.

View SoloHill Animal Product Free Microcarriers

Key Features and Benefits

  • Excellent attachment and growth across multiple cell lines
  • No hydration needed, simply sterilize and use.
  • Solid core design able to withstand mechanical harvest methods
  • Starter Kits available to test in your process

The flexibility of this family of microcarriers ensures that millions of grams of SoloHill collagen coated microcarriers are used annually for the production of biopharmaceutical products and animal vaccines.Two features contribute to this success:a micro layer of denatured collagen and a copolymer core.Despite the small amount of animal protein used, SoloHill collagen microcarriers demonstrate ideal attachment kinetics across a wide variety of tested cell lines.The solid core design ensures no carrier fragmentation during culture conditions or aggressive harvest protocols.The smooth surface prevents absorption of media components and ensures quick proteolysis for faster preparation of single cell suspensions.Viable cell yield typically exceeds 95% and cells perform well in subsequent inoculations.These carriers are an excellent choice for scale-up projects where process development timelines are limiting.

Collagen Coated

Collagen, a normal component of the extracellular matrix produced by attached cells, has been proven to be an effective promoter of cell attachment and growth for anchorage dependent cell lines.

Experience gained in conventional collagen monolayers is directly applicable to microcarriers.Most cell lines attach and grow rapidly on SoloHill collagen microcarriers.

  • Size range:90-150 microns
  • Specific gravity:1.02-1.05 and 1.30
  • Autoclavable

FACT III

Fast Attachment Collagen Treated (FACT) microcarriers utilize a proprietary surface modification of the collagen layer to enhance performance.Attachment kinetics are improved in comparison to standard collagen coated microcarriers while maintaining the same rapid growth.

  • Size range:90-150 microns
  • Specific gravity:1.02-1.05 and 1.30
  • Autoclavable

Animal Protein Coated Starter Kits

The best microcarrier and attachment conditions may vary between cell types; experimentation will allow the most confident selection of the optimal microcarrier.To accelerate your evaluation process, SoloHill microcarriers are available in a convenient starter kit.

The SoloHill Microcarrier Starter Kit makes it easy to design multivariate experiments which explore the performance of SoloHill microcarriers across media formulations and cell lines.This Standard Starter kit has been designed for general cell culture use with any anchorage-dependent cell line.

Each kit contains:35 grams of microcarriers - 5g each Hillex II, Plastic, Plastic Plus, Glass Coated, ProNectin F, Collagen Coated, and FACT III microcarriers.

技术支持

颇尔 Corporation technical support specialists can assist you in identifying the optimal microcarrier for your needs as well as provide example protocols to suit many process requirements.

Contacting technical support connects you to a worldwide network of microbiologists and cell culture scientists who are actively engaged in hands-on research and development.They can provide consultation on protocol development, microcarrier selection and industry trends.For those seeking more comprehensive support, training services are available focusing on microcarrier selection, process optimization, and more.Our support ensures experienced researchers and those using microcarriers for the first time are employing state of the art approaches and maximizing resources.
 
 

SoloHill® Microcarrier Applications

Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers.Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.

文章

  SoloHill Microcarriers in a 25-L PadReactorTM Single-Use Bioreactor
Originally published in BioProcess International, Industry Yearbook 2012-2013 - This article summarizes a case study demonstrating the unique performance attributes of Collagen microcarriers as a substrate for Vero cells in a PadReactor.In addition to positive growth characteristics, the data show cells derived from this high-density culture can be serially passaged to fresh microcarriers for efficient cell expansion.

  Single-Use Bioreactors and Microcarriers - Scalable Technology for Cell-Based Therapies
Originally published in BioProcess International, March 2014, BioProcess Technical Section - This article discusses the current challenges that must be addressed before commercialization of cell therapies can become a reality.Specific focus is given to expansion of cells on microcarriers in multiple single-use bioreactor formats and how this approach allows key quality attributes of cell based therapeutics to be maintained.

  Meeting Lot-Size Challenges of Manufacturing Adherent Cells for Therapy
Originally published in BioProcess International, March 2012, Cell Therapies Manufacturing Section - This article outlines the current technologies available for adherent cell expansion including planar surfaces, packed bed reactors and microcarriers.Features and challenges of each approach are detailed and the authors call for earlier consideration of necessary scale up in all cell therapy research.

Applications Notes

  Expansion of MDCK Cells on 颇尔 SoloHill® Microcarriers via Growth in Stirred Vessels
MDCK cells are a kidney epithelial cell line derived from a healthy adult female dog.These cells are currently employed for production of Influenza A and B viruses used in trivalent vaccines.In this application note, we characterize MDCK cell growth on four SoloHill microcarriers (Collagen, Fact III, Plastic Plus, Hillex II) in stirred vessels.

  颇尔 SoloHill® Microcarriers in the PadReactor™ Single-Use Bioreactor
The recent emergence of disposable bioreactor technology, such as the PadReactor bioreactor has provided a useful platform for the manufacture of biologics.In this application note we demonstrate the compatibility and performance of 颇尔 SoloHill Collagen microcarriers for expansion of Vero cells in a single-use PadReactor bioreactor.

  Expansion and Characterization of Mesenchymal Stem Cells on 颇尔 SoloHill® Microcarriers
Mesenchymal stem cells (MSCs) are self-renewing cells that differentiate into several terminally differentiated cell types.These cells have been isolated from multiple adult tissues.In this application note we characterize MSC expansion on five SoloHill microcarriers (Collagen, Plastic, Plastic Plus, Pronectin® F, Hillex® II) in stirred vessels and verify retention of multipotency.

  Expansion of MRC-5 Cells on 颇尔 SoloHill® Microcarriers via Serial Passage in Stirred Vessels
MRC-5 cells are a human-derived lung fibroblast diploid cell line, commonly used for production of vaccines such as rubella, hepatitis A, varicella, rabies, and smallpox.In this application note we characterize MRC-5 cell growth on multiple 颇尔 SoloHill microcarriers (Collagen, Hillex II, Fact III, Plastic Plus) in stirred vessels and present data demonstrating feasibility scale-up into bioreactors.

  Expansion of Mesenchymal Stem Cells on 颇尔 SoloHill® Microcarriers
Mesenchymal stem cells (MSCs) are self-renewing cells that differentiate into several terminally differentiated cell types.For many stem cell applications, large numbers of cells are needed and expansion must be optimized.In this application note we expand MSC cultures on Collagen microcarriers in spinner vessels to a 3L single-use bioreactor.

  SoloHill® Microcarriers Support Normal Growth of Stem Cells in the New Brunswick◆ Galaxy◆ 170 R Incubator
Human Mesenchymal Stem Cells (hMSCs)are known to be resistant to the toxicity of oxygen deprivation, and may even grow to higher densities under these conditions.In this application note the New Brunswick Galaxy 170 series incubator is used to culture hMSCs in a low oxygen environment on multiple 颇尔 SoloHill microcarriers (Collagen, Plastic, Pronectin F, Plastic Plus).Growth, morphology and differentiation potential are explored in comparison to normoxic conditions.

Researcher Reported Applications

 
Cell Line 描述 Glass Coated Plastic & Plastic Plus Collagen Coated Charged Collagen Coated
Vero monkey kidney X   X X
CEF chick embryo fibroblast X X    
BHK hamster kidney X      
ST swine testes X   X  
BT bovine turbinate     X  
P4RSF rabbit skin fibroblast X   X  
MRC-5 human diploid fibroblast X   X  
KB human epithelial carcinoma     X  
HFF human foreskin fibroblast X X X X
UMSCC human squamous carcinoma X X X X
MDBK Madin-Darby bovine kidney X X X  
CHO chinese hamster ovary     X  
MDCK Madin-Darby canine kidney     X  
 

Report your SoloHill microcarrier success story, Contact Us.

SoloHill Microcarriers for Therapeutics and Vaccines

SoloHill microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.

FDA-licensed human therapeutics:

  • tissue plasminogen activator
  • thyroid cancer diagnostic
  • thyroid cancer therapy
  • beta interferon – MS indication

USDA-licensed animal vaccines:

  • equine influenza
  • canine parvovirus
  • feline leukemia and many others

颇尔 SoloHill scientists are actively researching new applications.This research has led to completed development on novel human vaccine protocols.

  • Human influenza vaccine
  • Marek's disease vaccine
 

Standard Starter Kit

SK102-1521(B):Kit includes:5g each Hillex II, Plastic, Plastic Plus, Glass Coated, ProNectin F Coated, Collagen Coated, and FACT III microcarriers.

 
Series and Description Model # Relative Density Diameter (μm) Area (cm2) Surface Charge Drug Master File
Fact III cross-linked polystyrene coated with Type 1 porcine collagen (gelatin) containing protein, cationic charge F102-915 1.022-1.030 90-150 480 X MF6915
F102-1521 1.022-1.030 125-212 360 X
F104-1521 1.034-1.046 125-212 360 X
Collagen Coated cross-linked polystyrene coated with Type 1 porcine collagen (gelatin) containing protein C102-915 1.022-1.030 90-150 480   MF3092
C102-1521 1.022-1.030 125-212 360  
C104-1521 1.041-1.049 125-212 360  
 

SoloHill microcarriers are also available in a number of custom configurations

  • small diameters:30-90 microns
  • large diameters:212-3000 microns
  • relative densities:0.95-1.30
  • specialized colors and/or coating options

For more information, Contact Us

Total Quality System certified to ISO 9001:2008 and ISO 13485:2003 standards

Introduction to Microcarriers

颇尔 SoloHill Microcarrier Training Course is designed for experienced and novice cell culture scientists looking to take their process to the next level.Attendees are taken through a three day course designed to highlight process optimization of microcarrier culture attachment and growth conditions.

 

Training courses are fully customizable and can include any of the following areas of targeted instruction

  • Adapting flatware & roller bottle processes to microcarriers
  • Handling microcarriers & optimizing attachment conditions
  • Small scale microcarrier processes:spinner flasks
  • Glass Bioreactor microcarrier processes:2 – 15L
  • Single-Use microcarrier processes:2 – 20L
  • Process optimization strategies
  • Microscopic imaging techniques:fluorescent & phase contrast
  • Sterile handling techniques

Training courses are led by PhD and MS level scientists who regularly work with common attachment cell lines/types:Vero, MDCK, SK-RST, HEK293, CHO-K1, Bovine Testes, Mesenchymal Stem Cells, and several others.Course attendees may choose to work with a common cell type or provide their own for tailored training.

Course tuition varies based on cell type, number of attendees and course content.Additional training available at customer’s location following first round training at 颇尔 SoloHill.

Contact Us for more information on tailored microcarrier training.

 
系列 Model # Catalog # 重量
Collagen Coated C102-915 C-215-020 10g
    C-215-030 25g
    C-215-040 50g
    C-215-050 100g
    C-215-060 200g
    C-215-070 500g
    C-215-080 1000g
  C102-1521 C-221-020 10g
    C-221-030 25g
    C-221-040 50g
    C-221-050 100g
    C-221-060 200g
    C-221-070 500g
    C-221-080 1000g
  C104-1521 C-421-020 10g
    C-421-030 25g
    C-421-040 50g
    C-421-050 100g
    C-421-060 200g
    C-421-070 500g
    C-421-080 1000g
 Fact III  F102-915 F-215-020 10g
    F-215-030 25g
    F-215-040 50g
    F-215-050 100g
    F-215-060 200g
    F-215-070 500g
    F-215-080 1000g
  F102-152 F-221-020 10g
    F-221-030 25g
    F-221-040 50g
    F-221-050 100g
    F-221-060 200g
    F-221-070 500g
    F-221-080 1000g
  F104-1521 F-421-020 10g
    F-421-030 25g
    F-421-040 50g
    F-421-050 100g
    F-421-060 200g
    F-421-070 500g
    F-421-080 1000g
 

SoloHill microcarriers are also available in a number of custom configurations

  • small diameters:30-90 microns
  • large diameters:212-3000 microns
  • relative densities:0.95-1.30
  • specialized colors and/or coating options

For more information, Contact Us

 


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