Animal Protein Coated Microcarriers
Anchorage dependent cell lines rely heavily on optimal substrate conditions.Collagen has been shown to be an effective promoter of cell attachment and growth in monolayer cultures.With collagen coated microcarriers, processes can be transferred directly out of collagen based monolayers and moved into space efficient 3D cultures with minimal development time.This upgrade allows improved process control and process economics.
View SoloHill Animal Product Free Microcarriers
Key Features and Benefits
- Excellent attachment and growth across multiple cell lines
- No hydration needed, simply sterilize and use.
- Solid core design able to withstand mechanical harvest methods
- Starter Kits available to test in your process
The flexibility of this family of microcarriers ensures that millions of grams of SoloHill collagen coated microcarriers are used annually for the production of biopharmaceutical products and animal vaccines.Two features contribute to this success:a micro layer of denatured collagen and a copolymer core.Despite the small amount of animal protein used, SoloHill collagen microcarriers demonstrate ideal attachment kinetics across a wide variety of tested cell lines.The solid core design ensures no carrier fragmentation during culture conditions or aggressive harvest protocols.The smooth surface prevents absorption of media components and ensures quick proteolysis for faster preparation of single cell suspensions.Viable cell yield typically exceeds 95% and cells perform well in subsequent inoculations.These carriers are an excellent choice for scale-up projects where process development timelines are limiting.
Collagen Coated
Collagen, a normal component of the extracellular matrix produced by attached cells, has been proven to be an effective promoter of cell attachment and growth for anchorage dependent cell lines.
Experience gained in conventional collagen monolayers is directly applicable to microcarriers.Most cell lines attach and grow rapidly on SoloHill collagen microcarriers.
- Size range:90-150 microns
- Specific gravity:1.02-1.05 and 1.30
- Autoclavable
FACT III
Fast Attachment Collagen Treated (FACT) microcarriers utilize a proprietary surface modification of the collagen layer to enhance performance.Attachment kinetics are improved in comparison to standard collagen coated microcarriers while maintaining the same rapid growth.
- Size range:90-150 microns
- Specific gravity:1.02-1.05 and 1.30
- Autoclavable
Animal Protein Coated Starter Kits
The best microcarrier and attachment conditions may vary between cell types; experimentation will allow the most confident selection of the optimal microcarrier.To accelerate your evaluation process, SoloHill microcarriers are available in a convenient starter kit.
The SoloHill Microcarrier Starter Kit makes it easy to design multivariate experiments which explore the performance of SoloHill microcarriers across media formulations and cell lines.This Standard Starter kit has been designed for general cell culture use with any anchorage-dependent cell line.
Each kit contains:35 grams of microcarriers - 5g each Hillex II, Plastic, Plastic Plus, Glass Coated, ProNectin F, Collagen Coated, and FACT III microcarriers.
技术支持
颇尔 Corporation technical support specialists can assist you in identifying the optimal microcarrier for your needs as well as provide example protocols to suit many process requirements.
Contacting technical support connects you to a worldwide network of microbiologists and cell culture scientists who are actively engaged in hands-on research and development.They can provide consultation on protocol development, microcarrier selection and industry trends.For those seeking more comprehensive support, training services are available focusing on microcarrier selection, process optimization, and more.Our support ensures experienced researchers and those using microcarriers for the first time are employing state of the art approaches and maximizing resources. |
SoloHill® Microcarrier Applications
Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers.Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.文章
SoloHill Microcarriers in a 25-L PadReactorTM Single-Use Bioreactor |
Single-Use Bioreactors and Microcarriers - Scalable Technology for Cell-Based Therapies |
Meeting Lot-Size Challenges of Manufacturing Adherent Cells for Therapy |
Applications Notes
Expansion of MDCK Cells on 颇尔 SoloHill® Microcarriers via Growth in Stirred Vessels |
颇尔 SoloHill® Microcarriers in the PadReactor™ Single-Use Bioreactor |
Expansion and Characterization of Mesenchymal Stem Cells on 颇尔 SoloHill® Microcarriers |
Expansion of MRC-5 Cells on 颇尔 SoloHill® Microcarriers via Serial Passage in Stirred Vessels |
Expansion of Mesenchymal Stem Cells on 颇尔 SoloHill® Microcarriers |
SoloHill® Microcarriers Support Normal Growth of Stem Cells in the New Brunswick◆ Galaxy◆ 170 R Incubator |
Researcher Reported Applications
Cell Line | 描述 | Glass Coated | Plastic & Plastic Plus | Collagen Coated | Charged Collagen Coated |
Vero | monkey kidney | X | X | X | |
CEF | chick embryo fibroblast | X | X | ||
BHK | hamster kidney | X | |||
ST | swine testes | X | X | ||
BT | bovine turbinate | X | |||
P4RSF | rabbit skin fibroblast | X | X | ||
MRC-5 | human diploid fibroblast | X | X | ||
KB | human epithelial carcinoma | X | |||
HFF | human foreskin fibroblast | X | X | X | X |
UMSCC | human squamous carcinoma | X | X | X | X |
MDBK | Madin-Darby bovine kidney | X | X | X | |
CHO | chinese hamster ovary | X | |||
MDCK | Madin-Darby canine kidney | X | |||
Report your SoloHill microcarrier success story, Contact Us.
SoloHill Microcarriers for Therapeutics and Vaccines
SoloHill microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.
FDA-licensed human therapeutics:
- tissue plasminogen activator
- thyroid cancer diagnostic
- thyroid cancer therapy
- beta interferon – MS indication
USDA-licensed animal vaccines:
- equine influenza
- canine parvovirus
- feline leukemia and many others
颇尔 SoloHill scientists are actively researching new applications.This research has led to completed development on novel human vaccine protocols.
- Human influenza vaccine
- Marek's disease vaccine
Standard Starter Kit
SK102-1521(B):Kit includes:5g each Hillex II, Plastic, Plastic Plus, Glass Coated, ProNectin F Coated, Collagen Coated, and FACT III microcarriers.
Series and Description | Model # | Relative Density | Diameter (μm) | Area (cm2) | Surface Charge | Drug Master File |
Fact III cross-linked polystyrene coated with Type 1 porcine collagen (gelatin) containing protein, cationic charge | F102-915 | 1.022-1.030 | 90-150 | 480 | X | MF6915 |
F102-1521 | 1.022-1.030 | 125-212 | 360 | X | ||
F104-1521 | 1.034-1.046 | 125-212 | 360 | X | ||
Collagen Coated cross-linked polystyrene coated with Type 1 porcine collagen (gelatin) containing protein | C102-915 | 1.022-1.030 | 90-150 | 480 | MF3092 | |
C102-1521 | 1.022-1.030 | 125-212 | 360 | |||
C104-1521 | 1.041-1.049 | 125-212 | 360 | |||
SoloHill microcarriers are also available in a number of custom configurations
- small diameters:30-90 microns
- large diameters:212-3000 microns
- relative densities:0.95-1.30
- specialized colors and/or coating options
For more information, Contact Us
Total Quality System certified to ISO 9001:2008 and ISO 13485:2003 standards
Introduction to Microcarriers
颇尔 SoloHill Microcarrier Training Course is designed for experienced and novice cell culture scientists looking to take their process to the next level.Attendees are taken through a three day course designed to highlight process optimization of microcarrier culture attachment and growth conditions.
Training courses are fully customizable and can include any of the following areas of targeted instruction
- Adapting flatware & roller bottle processes to microcarriers
- Handling microcarriers & optimizing attachment conditions
- Small scale microcarrier processes:spinner flasks
- Glass Bioreactor microcarrier processes:2 – 15L
- Single-Use microcarrier processes:2 – 20L
- Process optimization strategies
- Microscopic imaging techniques:fluorescent & phase contrast
- Sterile handling techniques
Training courses are led by PhD and MS level scientists who regularly work with common attachment cell lines/types:Vero, MDCK, SK-RST, HEK293, CHO-K1, Bovine Testes, Mesenchymal Stem Cells, and several others.Course attendees may choose to work with a common cell type or provide their own for tailored training.
Course tuition varies based on cell type, number of attendees and course content.Additional training available at customer’s location following first round training at 颇尔 SoloHill.
Contact Us for more information on tailored microcarrier training.
系列 | Model # | Catalog # | 重量 | ||
Collagen Coated | C102-915 | C-215-020 | 10g | ||
C-215-030 | 25g | ||||
C-215-040 | 50g | ||||
C-215-050 | 100g | ||||
C-215-060 | 200g | ||||
C-215-070 | 500g | ||||
C-215-080 | 1000g | ||||
C102-1521 | C-221-020 | 10g | |||
C-221-030 | 25g | ||||
C-221-040 | 50g | ||||
C-221-050 | 100g | ||||
C-221-060 | 200g | ||||
C-221-070 | 500g | ||||
C-221-080 | 1000g | ||||
C104-1521 | C-421-020 | 10g | |||
C-421-030 | 25g | ||||
C-421-040 | 50g | ||||
C-421-050 | 100g | ||||
C-421-060 | 200g | ||||
C-421-070 | 500g | ||||
C-421-080 | 1000g | ||||
Fact III | F102-915 | F-215-020 | 10g | ||
F-215-030 | 25g | ||||
F-215-040 | 50g | ||||
F-215-050 | 100g | ||||
F-215-060 | 200g | ||||
F-215-070 | 500g | ||||
F-215-080 | 1000g | ||||
F102-152 | F-221-020 | 10g | |||
F-221-030 | 25g | ||||
F-221-040 | 50g | ||||
F-221-050 | 100g | ||||
F-221-060 | 200g | ||||
F-221-070 | 500g | ||||
F-221-080 | 1000g | ||||
F104-1521 | F-421-020 | 10g | |||
F-421-030 | 25g | ||||
F-421-040 | 50g | ||||
F-421-050 | 100g | ||||
F-421-060 | 200g | ||||
F-421-070 | 500g | ||||
F-421-080 | 1000g | ||||
SoloHill microcarriers are also available in a number of custom configurations
- small diameters:30-90 microns
- large diameters:212-3000 microns
- relative densities:0.95-1.30
- specialized colors and/or coating options
For more information, Contact Us