pall,颇尔,SoloHill 微载体 – 动物产品免费,HCT-170-045, HCT-170-075, H-170-020, H-170-030, H-170-040, H-170-050, H-170-060, H-170-070, H-170-080, G-221-020, G-221-030, G-221-040, G-221-050, G-221-060, G-221-070, P

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SoloHill 微载体 – 动物产品免费
产品型号:
HCT-170-045, HCT-170-075, H-170-020, H-170-030, H-170-040, H-170-050, H-170-060, H-170-070, H-170-080, G-221-020, G-221-030, G-221-040, G-221-050, G-221-060, G-221-070, PF-221-020, PF-221-030, PF-221-040, PF-221-050, PF-221-060, PF-221-070, PF-221-08
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产品介绍

pall,颇尔,SoloHill 微载体 – 动物产品免费,HCT-170-045, HCT-170-075, H-170-020, H-170-030, H-170-040, H-170-050, H-170-060, H-170-070, H-170-080, G-221-020, G-221-030, G-221-040, G-221-050, G-221-060, G-221-070, P
pall,颇尔,SoloHill 微载体 – 动物产品免费

SoloHill 微载体 – 动物产品免费

Animal Product Free Microcarriers

SoloHill Animal Product Free Microcarriers offer high performance options for researchers wishing to limit or remove animal sources from their processes.They address the worldwide concern with the spread of bovine spongiform encephalopathy (BSE) from cattle to humans as vCJD (variant Creutzfeldt-Jakob disease) which has driven the world's regulatory agencies to strongly suggest the eventual removal of all animal proteins from pharmaceutical manufacturing processes.

View SoloHill Animal Protein Coated Microcarriers

Key Features and Benefits

  • Raw materials verified Animal Product Free
  • Pairs well with serum free and animal free media
  • Five different Animal Product Free Microcarriers available
  • Starter Kits available to test in your process

The biopharmaceutical and cell therapy industry have expressed their desire to combine serum free media and animal component derivative free (ACDF) microcarriers in protocols for complete manufacturing processes.SoloHill microcarriers offer an immediate step toward this goal.Available now, SoloHill microcarriers can be paired with existing processes and newly developed ACDF media formulations.

The shift to animal free processes is ongoing, requiring collaboration between media manufacturers, microcarrier manufacturers and researchers.颇尔 Corporation is dedicated to this goal and works closely with media manufacturers and researchers to optimize processes and further develop Animal Product Free Microcarriers.

Hillex® and Hillex®CT

The polystyrene core of the Hillex family of microcarriers is modified with a cationic amine which improves cell attraction.A unique microporous surface improves attachment without affecting harvest efficiency.Hillex CT features the lowest particle counts for a microcarrier of its kind and is particularly well suited for cell therapy applications

  • Size range:160-200 microns
  • Specific gravity:1.09-1.15
  • Autoclavable

Plastic and Plastic Plus

These microcarriers feature a styrene copolymer for easy process transfer out of traditional styrene labware methods such as roller bottles and monolayers.Plastic Plus beads incorporate an electric charge to enhance cell attraction and attachment.Solid construction from non-toxic plastic prevents absorption of toxic byproducts.Harvesting is simplified compared to porous microcarriers and harvest cell viabilities are typically greater than 95%.

  • Size range:90-150 micron, 125-212 micron
  • Specific gravity:1.02-1.05, 1.30
  • Autoclavable

Glass Coated

Glass has been used as a substrate for cell and tissue culture for many years in roller bottles and monolayer cultures.However, solid glass is poorly suited to microcarrier construction due to its high density (2.4g/cm3). SoloHill microcarriers feature a glass coating on copolymer plastic spheres, limiting density while providing a high silica glass attachment surface.Solid construction prevents absorption of toxic byproducts.Harvesting is simplified compared to porous microcarriers and when proteolytic methods are used viability greater than 95% is typical.The robust design of this carrier also allows harvest methods such as ultrasonication and free thaw.

  • Size range:125-212 micron
  • Specific gravity:1.02-1.05, 1.30
  • Autoclavable

Animal Product Free Starter Kits

The best microcarrier and attachment conditions may vary between cell types; experimentation will allow the most confident selection of the optimal microcarrier.To accelerate your evaluation process, SoloHill microcarriers are available in a convenient starter kit.

The SoloHill Microcarrier Starter Kit makes it easy to design multivariate experiments which explore the performance of SoloHill microcarriers across media formulations and cell lines.This Animal Product Free kit has been designed for general cell culture use with any anchorage-dependent cell line.

Each kit contains:35 grams of microcarriers - 10 g each Hillex II, Plastic Plus, and 5 g each Plastic, ProNectin F-Coated and Glass-Coated microcarriers.

技术支持

SoloHill microcarriers are the most complete line of Animal Product Free microcarriers available to address regulatory matters and today's market requirements.颇尔 Corporation technical support specialists can assist you in identifying the optimal microcarrier for your needs as well as provide example protocols to suit many process requirements.

Contacting technical support connects you to a worldwide network of microbiologists and cell culture scientists who are actively engaged in hands-on research and development.They can provide consultation on protocol development, microcarrier selection and industry trends.For those seeking more comprehensive support, training services are available focusing on microcarrier selection, process optimization, and more.Our support ensures experienced researchers and those using microcarriers for the first time are employing state of the art approaches and maximizing resources.
 
 

SoloHill® Microcarrier Applications

Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers.Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.

文章

  Single-Use Bioreactors and Microcarriers - Scalable Technology for Cell-Based Therapies
Originally published in BioProcess International, March 2014, BioProcess Technical Section - This article discusses the current challenges that must be addressed before commercialization of cell therapies can become a reality.Specific focus is given to expansion of cells on microcarriers in multiple single-use bioreactor formats and how this approach allows key quality attributes of cell based therapeutics to be maintained.

  Meeting Lot-Size Challenges of Manufacturing Adherent Cells for Therapy
Originally published in BioProcess International, March 2012, Cell Therapies Manufacturing Section - This article outlines the current technologies available for adherent cell expansion including planar surfaces, packed bed reactors and microcarriers.Features and challenges of each approach are detailed and the authors call for earlier consideration of necessary scale up in all cell therapy research.

Applications Notes

  Expansion of Porcine SK-RST Cells on Hillex® II Microcarriers via Serial Passage in Stirred Vessels
Porcine SK-RST kidney epithelial cells are commonly used for diagnostic testing of swine diseases and for production of veterinary and human pharmaceuticals.In this application note we present data demonstrating the feasibility of serial passaging porcine SK-RST cells on Hillex II microcarriers in stirred vessels.

  Expansion of MDCK Cells on 颇尔 SoloHill® Microcarriers via Growth in Stirred Vessels
MDCK cells are a kidney epithelial cell line derived from a healthy adult female dog.These cells are currently employed for production of Influenza A and B viruses used in trivalent vaccines.In this application note, we characterize MDCK cell growth on four SoloHill microcarriers (Collagen, Fact III, Plastic Plus, Hillex II) in stirred vessels.

  Expansion of Vero Cells on Hillex® II Microcarriers via Serial Passage in Stirred Vessels
Vero cells are a kidney epithelial cell line isolated from a Chlorocebus monkey.They are commonly used in veterinary and human medicine.In this application note we present data demonstrating feasibility of serial passaging Vero cells on Hillex II microcarriers in bioreactors.Three serial passages are performed proceeding from a 0.2 L spinner through a 10 L culture in a bioreactor.

  Expansion and Characterization of Mesenchymal Stem Cells on 颇尔 SoloHill® Microcarriers
Mesenchymal stem cells (MSCs) are self-renewing cells that differentiate into several terminally differentiated cell types.These cells have been isolated from multiple adult tissues.In this application note we characterize MSC expansion on five SoloHill microcarriers (Collagen, Plastic, Plastic Plus, Pronectin® F, Hillex® II) in stirred vessels and verify retention of multipotency.

  Expansion of MRC-5 Cells on 颇尔 SoloHill® Microcarriers via Serial Passage in Stirred Vessels
MRC-5 cells are a human-derived lung fibroblast diploid cell line, commonly used for production of vaccines such as rubella, hepatitis A, varicella, rabies, and smallpox.In this application note we characterize MRC-5 cell growth on multiple 颇尔 SoloHill microcarriers (Collagen, Hillex II, Fact III, Plastic Plus) in stirred vessels and present data demonstrating feasibility scale-up into bioreactors.

  SoloHill® Microcarriers Support Normal Growth of Stem Cells in the New Brunswick◆ Galaxy◆ 170 R Incubator
Human Mesenchymal Stem Cells (hMSCs)are known to be resistant to the toxicity of oxygen deprivation, and may even grow to higher densities under these conditions.In this application note the New Brunswick Galaxy 170 series incubator is used to culture hMSCs in a low oxygen environment on multiple 颇尔 SoloHill microcarriers (Collagen, Plastic, Pronectin F, Plastic Plus).Growth, morphology and differentiation potential are explored in comparison to normoxic conditions.

Researcher Reported Applications

 
Cell Line 描述 Glass Coated Plastic & Plastic Plus Collagen Coated Charged Collagen Coated
Vero monkey kidney X   X X
CEF chick embryo fibroblast X X    
BHK hamster kidney X      
ST swine testes X   X  
BT bovine turbinate     X  
P4RSF rabbit skin fibroblast X   X  
MRC-5 human diploid fibroblast X   X  
KB human epithelial carcinoma     X  
HFF human foreskin fibroblast X X X X
UMSCC human squamous carcinoma X X X X
MDBK Madin-Darby bovine kidney X X X  
CHO chinese hamster ovary     X  
MDCK Madin-Darby canine kidney     X  
 

Report your SoloHill microcarrier success story, Contact Us.

SoloHill Microcarriers for Therapeutics and Vaccines

SoloHill microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.

FDA-licensed human therapeutics:

  • tissue plasminogen activator
  • thyroid cancer diagnostic
  • thyroid cancer therapy
  • beta interferon – MS indication

USDA-licensed animal vaccines:

  • equine influenza
  • canine parvovirus
  • feline leukemia and many others

颇尔 SoloHill scientists are actively researching new applications.This research has led to completed development on novel human vaccine protocols.

  • Human influenza vaccine
  • Marek's disease vaccine
 

Animal Product Free (APF) Starter Kit

SK102-1521(APF):Kit Includes:10 g each Hillex II, Plastic Plus, and 5 g each Plastic, ProNectin F-Coated and Glass-Coated microcarriers.

Animal Product Free (APF) Microcarriers

 
Series and Description Model # Relative
Density
Diameter
(μm)
Area
(cm2)
Surface
Charge
Drug
Master File
Hillex®CT
virtually particle-free modified polystyrene, supplied wetted
HCT112 - 170 1.090-1.150 160-200 257 X MF10059
Hillex®II
modified polystyrene
H112 - 170 1.090-1.150 160-200 515 X MF10059
Plastic cross-linked polystyrene P102-915 1.022-1.030 90-150 480   MF 3094
P102-1521 1.022-1.030 125-212 360   MF 3094
P104-1521 1.041-1.049 125-212 360   MF 3094
Plastic Plus
cross-linked polystyrene, cationic charge
PP102-915 1.022-1.030 90-150 480 X MF10600
PP102-1521 1.022-1.030 125-212 360 X MF10600
PP104-1521 1.041-1.049 125-212 360 X MF10600
Glass Coated
cross-linked polystyrene coated with high silica glass
G102-1521 1.022-1.030 125-212 360   MF3093
Pronectin F
cross-linked polystyrene coated with recombinant RGD-containing protein
PF102-915 1.022-1.030 90-150 480 X MF6915
PF102-1521 1.022-1.030 125-212 360 X MF6915
PF104-1521 1.041-1.049 125-212 360 X MF6915
 

 

SoloHill microcarriers are also available in a number of custom configurations

  • small diameters:30-90 microns
  • large diameters:212-3000 microns
  • relative densities:0.95-1.30
  • specialized colors and/or coating options
  • gamma irradiated

For more information, Contact Us

Total Quality System certified to ISO 9001:2008 and ISO 13485:2003 standards

Introduction to Microcarriers

颇尔 SoloHill Microcarrier Training Course is designed for experienced and novice cell culture scientists looking to take their process to the next level.Attendees are taken through a three day course designed to highlight process optimization of microcarrier culture attachment and growth conditions.

 

Training courses are fully customizable and can include any of the following areas of targeted instruction

  • Adapting flatware & roller bottle processes to microcarriers
  • Handling microcarriers & optimizing attachment conditions
  • Small scale microcarrier processes:spinner flasks
  • Glass Bioreactor microcarrier processes:2 – 15L
  • Single-Use microcarrier processes:2 – 20L
  • Process optimization strategies
  • Microscopic imaging techniques:fluorescent & phase contrast
  • Sterile handling techniques

Training courses are led by PhD and MS level scientists who regularly work with common attachment cell lines/types:Vero, MDCK, SK-RST, HEK293, CHO-K1, Bovine Testes, Mesenchymal Stem Cells, and several others.Course attendees may choose to work with a common cell type or provide their own for tailored training.

Course tuition varies based on cell type, number of attendees and course content.Additional training available at customer’s location following first round training at 颇尔 SoloHill.

Contact Us for more information on tailored microcarrier training.

 
系列 Model # Catalog # 重量
Hillex®CT HCT112 - 170 HCT-170-045 60g
    HCT-170-075 600g
Hillex®II H112 - 170 H-170-020 10g
    H-170-030 25g
    H-170-040 50g
    H-170-050 100g
    H-170-060 200g
    H-170-070 500g
    H-170-080 1000g
Glass Coated G102-1521 G-221-020 10g
    G-221-030 25g
    G-221-040 50g
    G-221-050 100g
    G-221-060 200g
    G-221-070 500g
Pronectin F PF102-1521 PF-221-020 10g
    PF-221-030 25g
    PF-221-040 50g
    PF-221-050 100g
    PF-221-060 200g
    PF-221-070 500g
    PF-221-080 1000g
Plastic P102-915 P-215-020 10g
    P-215-030 25g
    P-215-040 50g
    P-215-050 100g
    P-215-060 200g
    P-215-070 500g
    P-215-080 1000 g
  P102-1521 P-221-020 10g
    P-221-030 25g
    P-221-040 50g
    P-221-050 100g
    P-221-060 200g
    P-221-070 500g
    P-221-080 1000g
  P104-1521 P-421-020 10g
    P-421-030 25g
    P-421-040 50g
    P-421-050 100g
    P-421-060 200g
    P-421-070 500g
    P-421-080 1000g
Plastic Plus PP102-915 PP-215-020 10g
    PP-215-030 25g
    PP-215-040 50g
    PP-215-050 100g
    PP-215-060 200g
    PP-215-070 500g
    PP215-080 1000g
  PP102-1521 PP-221-020 10g
    PP-221-030 25g
    PP-221-040 50g
    PP-221-050 100g
    PP-221-060 200g
    PP-221-070 500g
    PP-221-080 1000g
  PP104-1521 PP-421-020 10g
    PP-421-030 25g
    PP-421-040 50g
    PP-421-050 100g
    PP-421-060 200g
    PP-421-070 500g
    PP-421-080 1000g
 

SoloHill microcarriers are also available in a number of custom configurations

  • small diameters:30-90 microns
  • large diameters:212-3000 microns
  • relative densities:0.95-1.30
  • specialized colors and/or coating options

For more information, Contact Us

 


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