Animal Product Free Microcarriers
SoloHill Animal Product Free Microcarriers offer high performance options for researchers wishing to limit or remove animal sources from their processes.They address the worldwide concern with the spread of bovine spongiform encephalopathy (BSE) from cattle to humans as vCJD (variant Creutzfeldt-Jakob disease) which has driven the world's regulatory agencies to strongly suggest the eventual removal of all animal proteins from pharmaceutical manufacturing processes.
View SoloHill Animal Protein Coated Microcarriers
Key Features and Benefits
- Raw materials verified Animal Product Free
- Pairs well with serum free and animal free media
- Five different Animal Product Free Microcarriers available
- Starter Kits available to test in your process
The biopharmaceutical and cell therapy industry have expressed their desire to combine serum free media and animal component derivative free (ACDF) microcarriers in protocols for complete manufacturing processes.SoloHill microcarriers offer an immediate step toward this goal.Available now, SoloHill microcarriers can be paired with existing processes and newly developed ACDF media formulations.
The shift to animal free processes is ongoing, requiring collaboration between media manufacturers, microcarrier manufacturers and researchers.颇尔 Corporation is dedicated to this goal and works closely with media manufacturers and researchers to optimize processes and further develop Animal Product Free Microcarriers.
Hillex® and Hillex®CT
The polystyrene core of the Hillex family of microcarriers is modified with a cationic amine which improves cell attraction.A unique microporous surface improves attachment without affecting harvest efficiency.Hillex CT features the lowest particle counts for a microcarrier of its kind and is particularly well suited for cell therapy applications
- Size range:160-200 microns
- Specific gravity:1.09-1.15
- Autoclavable
Plastic and Plastic Plus
These microcarriers feature a styrene copolymer for easy process transfer out of traditional styrene labware methods such as roller bottles and monolayers.Plastic Plus beads incorporate an electric charge to enhance cell attraction and attachment.Solid construction from non-toxic plastic prevents absorption of toxic byproducts.Harvesting is simplified compared to porous microcarriers and harvest cell viabilities are typically greater than 95%.
- Size range:90-150 micron, 125-212 micron
- Specific gravity:1.02-1.05, 1.30
- Autoclavable
Glass Coated
Glass has been used as a substrate for cell and tissue culture for many years in roller bottles and monolayer cultures.However, solid glass is poorly suited to microcarrier construction due to its high density (2.4g/cm3). SoloHill microcarriers feature a glass coating on copolymer plastic spheres, limiting density while providing a high silica glass attachment surface.Solid construction prevents absorption of toxic byproducts.Harvesting is simplified compared to porous microcarriers and when proteolytic methods are used viability greater than 95% is typical.The robust design of this carrier also allows harvest methods such as ultrasonication and free thaw.
- Size range:125-212 micron
- Specific gravity:1.02-1.05, 1.30
- Autoclavable
Animal Product Free Starter Kits
The best microcarrier and attachment conditions may vary between cell types; experimentation will allow the most confident selection of the optimal microcarrier.To accelerate your evaluation process, SoloHill microcarriers are available in a convenient starter kit.
The SoloHill Microcarrier Starter Kit makes it easy to design multivariate experiments which explore the performance of SoloHill microcarriers across media formulations and cell lines.This Animal Product Free kit has been designed for general cell culture use with any anchorage-dependent cell line.
Each kit contains:35 grams of microcarriers - 10 g each Hillex II, Plastic Plus, and 5 g each Plastic, ProNectin F-Coated and Glass-Coated microcarriers.
技术支持
SoloHill microcarriers are the most complete line of Animal Product Free microcarriers available to address regulatory matters and today's market requirements.颇尔 Corporation technical support specialists can assist you in identifying the optimal microcarrier for your needs as well as provide example protocols to suit many process requirements.
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Contacting technical support connects you to a worldwide network of microbiologists and cell culture scientists who are actively engaged in hands-on research and development.They can provide consultation on protocol development, microcarrier selection and industry trends.For those seeking more comprehensive support, training services are available focusing on microcarrier selection, process optimization, and more.Our support ensures experienced researchers and those using microcarriers for the first time are employing state of the art approaches and maximizing resources. |
SoloHill® Microcarrier Applications
Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers.Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.文章
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Single-Use Bioreactors and Microcarriers - Scalable Technology for Cell-Based Therapies |
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Meeting Lot-Size Challenges of Manufacturing Adherent Cells for Therapy |
Applications Notes
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Expansion of Porcine SK-RST Cells on Hillex® II Microcarriers via Serial Passage in Stirred Vessels |
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Expansion of MDCK Cells on 颇尔 SoloHill® Microcarriers via Growth in Stirred Vessels |
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Expansion of Vero Cells on Hillex® II Microcarriers via Serial Passage in Stirred Vessels |
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Expansion and Characterization of Mesenchymal Stem Cells on 颇尔 SoloHill® Microcarriers |
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Expansion of MRC-5 Cells on 颇尔 SoloHill® Microcarriers via Serial Passage in Stirred Vessels |
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SoloHill® Microcarriers Support Normal Growth of Stem Cells in the New Brunswick◆ Galaxy◆ 170 R Incubator |
Researcher Reported Applications
| Cell Line | 描述 | Glass Coated | Plastic & Plastic Plus | Collagen Coated | Charged Collagen Coated |
| Vero | monkey kidney | X | X | X | |
| CEF | chick embryo fibroblast | X | X | ||
| BHK | hamster kidney | X | |||
| ST | swine testes | X | X | ||
| BT | bovine turbinate | X | |||
| P4RSF | rabbit skin fibroblast | X | X | ||
| MRC-5 | human diploid fibroblast | X | X | ||
| KB | human epithelial carcinoma | X | |||
| HFF | human foreskin fibroblast | X | X | X | X |
| UMSCC | human squamous carcinoma | X | X | X | X |
| MDBK | Madin-Darby bovine kidney | X | X | X | |
| CHO | chinese hamster ovary | X | |||
| MDCK | Madin-Darby canine kidney | X | |||
Report your SoloHill microcarrier success story, Contact Us.
SoloHill Microcarriers for Therapeutics and Vaccines
SoloHill microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.
FDA-licensed human therapeutics:
- tissue plasminogen activator
- thyroid cancer diagnostic
- thyroid cancer therapy
- beta interferon – MS indication
USDA-licensed animal vaccines:
- equine influenza
- canine parvovirus
- feline leukemia and many others
颇尔 SoloHill scientists are actively researching new applications.This research has led to completed development on novel human vaccine protocols.
- Human influenza vaccine
- Marek's disease vaccine
Animal Product Free (APF) Starter Kit
SK102-1521(APF):Kit Includes:10 g each Hillex II, Plastic Plus, and 5 g each Plastic, ProNectin F-Coated and Glass-Coated microcarriers.
Animal Product Free (APF) Microcarriers
| Series and Description | Model # |
Relative Density |
Diameter (μm) |
Area (cm2) |
Surface Charge |
Drug Master File |
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Hillex®CT virtually particle-free modified polystyrene, supplied wetted |
HCT112 - 170 | 1.090-1.150 | 160-200 | 257 | X | MF10059 |
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Hillex®II modified polystyrene |
H112 - 170 | 1.090-1.150 | 160-200 | 515 | X | MF10059 |
| Plastic cross-linked polystyrene | P102-915 | 1.022-1.030 | 90-150 | 480 | MF 3094 | |
| P102-1521 | 1.022-1.030 | 125-212 | 360 | MF 3094 | ||
| P104-1521 | 1.041-1.049 | 125-212 | 360 | MF 3094 | ||
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Plastic Plus cross-linked polystyrene, cationic charge |
PP102-915 | 1.022-1.030 | 90-150 | 480 | X | MF10600 |
| PP102-1521 | 1.022-1.030 | 125-212 | 360 | X | MF10600 | |
| PP104-1521 | 1.041-1.049 | 125-212 | 360 | X | MF10600 | |
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Glass Coated cross-linked polystyrene coated with high silica glass |
G102-1521 | 1.022-1.030 | 125-212 | 360 | MF3093 | |
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Pronectin F cross-linked polystyrene coated with recombinant RGD-containing protein |
PF102-915 | 1.022-1.030 | 90-150 | 480 | X | MF6915 |
| PF102-1521 | 1.022-1.030 | 125-212 | 360 | X | MF6915 | |
| PF104-1521 | 1.041-1.049 | 125-212 | 360 | X | MF6915 | |
SoloHill microcarriers are also available in a number of custom configurations
- small diameters:30-90 microns
- large diameters:212-3000 microns
- relative densities:0.95-1.30
- specialized colors and/or coating options
- gamma irradiated
For more information, Contact Us
Total Quality System certified to ISO 9001:2008 and ISO 13485:2003 standards
Introduction to Microcarriers
颇尔 SoloHill Microcarrier Training Course is designed for experienced and novice cell culture scientists looking to take their process to the next level.Attendees are taken through a three day course designed to highlight process optimization of microcarrier culture attachment and growth conditions.
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Training courses are fully customizable and can include any of the following areas of targeted instruction
- Adapting flatware & roller bottle processes to microcarriers
- Handling microcarriers & optimizing attachment conditions
- Small scale microcarrier processes:spinner flasks
- Glass Bioreactor microcarrier processes:2 – 15L
- Single-Use microcarrier processes:2 – 20L
- Process optimization strategies
- Microscopic imaging techniques:fluorescent & phase contrast
- Sterile handling techniques
Training courses are led by PhD and MS level scientists who regularly work with common attachment cell lines/types:Vero, MDCK, SK-RST, HEK293, CHO-K1, Bovine Testes, Mesenchymal Stem Cells, and several others.Course attendees may choose to work with a common cell type or provide their own for tailored training.
Course tuition varies based on cell type, number of attendees and course content.Additional training available at customer’s location following first round training at 颇尔 SoloHill.
Contact Us for more information on tailored microcarrier training.
| 系列 | Model # | Catalog # | 重量 |
| Hillex®CT | HCT112 - 170 | HCT-170-045 | 60g |
| HCT-170-075 | 600g | ||
| Hillex®II | H112 - 170 | H-170-020 | 10g |
| H-170-030 | 25g | ||
| H-170-040 | 50g | ||
| H-170-050 | 100g | ||
| H-170-060 | 200g | ||
| H-170-070 | 500g | ||
| H-170-080 | 1000g | ||
| Glass Coated | G102-1521 | G-221-020 | 10g |
| G-221-030 | 25g | ||
| G-221-040 | 50g | ||
| G-221-050 | 100g | ||
| G-221-060 | 200g | ||
| G-221-070 | 500g | ||
| Pronectin F | PF102-1521 | PF-221-020 | 10g |
| PF-221-030 | 25g | ||
| PF-221-040 | 50g | ||
| PF-221-050 | 100g | ||
| PF-221-060 | 200g | ||
| PF-221-070 | 500g | ||
| PF-221-080 | 1000g | ||
| Plastic | P102-915 | P-215-020 | 10g |
| P-215-030 | 25g | ||
| P-215-040 | 50g | ||
| P-215-050 | 100g | ||
| P-215-060 | 200g | ||
| P-215-070 | 500g | ||
| P-215-080 | 1000 g | ||
| P102-1521 | P-221-020 | 10g | |
| P-221-030 | 25g | ||
| P-221-040 | 50g | ||
| P-221-050 | 100g | ||
| P-221-060 | 200g | ||
| P-221-070 | 500g | ||
| P-221-080 | 1000g | ||
| P104-1521 | P-421-020 | 10g | |
| P-421-030 | 25g | ||
| P-421-040 | 50g | ||
| P-421-050 | 100g | ||
| P-421-060 | 200g | ||
| P-421-070 | 500g | ||
| P-421-080 | 1000g | ||
| Plastic Plus | PP102-915 | PP-215-020 | 10g |
| PP-215-030 | 25g | ||
| PP-215-040 | 50g | ||
| PP-215-050 | 100g | ||
| PP-215-060 | 200g | ||
| PP-215-070 | 500g | ||
| PP215-080 | 1000g | ||
| PP102-1521 | PP-221-020 | 10g | |
| PP-221-030 | 25g | ||
| PP-221-040 | 50g | ||
| PP-221-050 | 100g | ||
| PP-221-060 | 200g | ||
| PP-221-070 | 500g | ||
| PP-221-080 | 1000g | ||
| PP104-1521 | PP-421-020 | 10g | |
| PP-421-030 | 25g | ||
| PP-421-040 | 50g | ||
| PP-421-050 | 100g | ||
| PP-421-060 | 200g | ||
| PP-421-070 | 500g | ||
| PP-421-080 | 1000g | ||
SoloHill microcarriers are also available in a number of custom configurations
- small diameters:30-90 microns
- large diameters:212-3000 microns
- relative densities:0.95-1.30
- specialized colors and/or coating options
For more information, Contact Us
