Fast and Efficient Detergent Removal
in a Convenient Column Format
- High dynamic binding capacity facilitates fast and efficient removal of many different detergents
- Columns come with luer inlet and outlet to simplify use with a syringe
- Provides high recovery of proteins
- Offers high adsorption capacity for small hydrophobic molecules
- Stable in acidic, polar organic, and oxidizing solutions
- Ideal for fast and simple removal of various (anionic, cationic, and nonionic) detergents from protein samples
- Removes SDS for improved enzymatic activity during protein digestion
- Removes detergents commonly used in membrane solubilization
材料成分
- Column Housing, Cap, Plug, and Adapter:聚丙烯
- Column Frit:聚乙烯
介质
- SDR HyperD
Color Code
- 自然
Average Particle Size
- 70 微米
Working pH
- 2 - 12
能力
- > 90 mg/mL1
Column Geometry
- Column Volume:1.04 毫升
- Bed Height:1.48 cm (0.58 in.)
- Bed Diameter:0.94 cm (0.37 in.)
Device Dimensions
- 直径：1.6 cm (0.6 in.)
- Length (Without Plugs):4.8 cm (1.9 in.)
Connections
- Inlet:Threaded female luer lock
- Outlet:Rotating male luer locking hub
Recommended Flow Rates
- 1 - 5 mL/min
Back Pressure
- Maximum:3 bar (300 kPa, 43.5 psi)
存储
- 2 - 30 °C (36 - 86 °F)
- 2 - 8 °C (36 - 46 °F) after opening
1DBC determined using 5 mg/mL Triton♦ X-100 at flow rate 3.5 mL/min (300 cm/hr) in PBS pH 7.2.
Detergent Removal Efficiency and Protein Recovery
| Detergent | % Removal Efficiency | % Protein Recovery |
| Triton X-100 | 100 | > 96 |
| NP 40 | > 99 | > 86 |
| SDS | > 86 | > 94 |
| Tween♦ 20 | 100 | > 98 |
| CHAPS | > 98 | 99 |
Data generated by syringe method using 0.5% detergent protein concentration at 2 mL/min with 2.5 mL injection.Protein recovery is measured by BCA assay or at 280 nm.
