pall,颇尔,AcroSep 亲合层析柱,20078-C001, 25896-C001

产品名称:
AcroSep 亲合层析柱
产品型号:
20078-C001, 25896-C001
产品描述:

产品介绍

pall,颇尔,AcroSep 亲合层析柱,20078-C001, 25896-C001
pall,颇尔,AcroSep 亲合层析柱

AcroSep 亲合层析柱

For the Purification of a Wide
Variety of Proteins and Antibodies

蛋白质 A 陶瓷 HyperD F 填料

  • Achieves > 90% recovery of IgG in a single step
  • Recombinant Protein A immobilized to specially formulated hydrogel within a ceramic bead offers high selectivity with low non-specific binding and low levels of Protein A leaching

Blue Trisacryl® M Sorbent

  • Trisacryl macroporous base matrix allows good diffusion and improves exchange kinetics
  • Cibacron blue dye ligand selective for enzymes and albumin
  • Enhanced stability due to ligand coupling mechanism prevents dye leakage
 
 

蛋白质 A 陶瓷 HyperD F 填料

  • Designed for IgG affinity purification from multiple sample types
  • Purification of Fc fusion and Ab-like proteins
  • Depletion of IgG from plasma and serum

蓝色Trisacryl M 填料

  • Optimized for depletion of albumin from plasma and serum samples
  • Good for purification of some enzymes
 
 

材料成分

  • Column Housing, Cap, Plug, and Adapter:聚丙烯
  • Column Frit:聚乙烯

 

 
介质 颜色 Average Particle Size Working pH 能力
蛋白质 A Ceramic HyperD F 珍珠白 50 微米 2 - 11 > 30 mg/mL1
Blue Trisacryl M 深蓝色 60 微米 1 - 10 10 - 15 mg/mL2
 

 

Column Geometry

  • Column Volume:1.04 毫升
  • Bed Height:1.48 cm (0.58 in.)
  • Bed Diameter:0.94 cm (0.37 in.)

 

Device Dimensions

  • 直径:1.6 cm (0.6 in.)
  • Length (Without Plugs):4.8 cm (1.9 in.)

 

Connections

  • Inlet:Threaded female luer lock
  • Outlet:Rotating male luer locking hub

 

Recommended Flow Rates

  • Protein A Ceramic HyperD F:0.2 - 1 mL/min
  • Blue Trisacryl M:0.2 - 1.0 mL/min

 

Back Pressure

  • Maximum:3 bar (300 kPa, 43.5 psi)

 

存储

  • 2 - 8 ºC (36 - 46 ºC)

 

110% breakthrough, 100 cm/hr, determined using 10 mg/mL human IgG in PBS, pH 7.4, elution in 0.1 M sodium citrate, pH 2.5, column 4.6 ID x 100 mm.
2Binding capacity using 5 mg/mL human albumin in PBS.Column dimensions – 1.6 cm ID x 3 cm bed height; residence time (Tr) = 7.26 min.
 
 

AcroSep™ Columns Provide Greater Capacity of IgG Compared to Competitive Columns


AcroSep Columns Provide Greater Capacity of IgG Compared to Competitive Columns

Recovery of IgG from human plasma quantified by ELISA.Data shows higher recovery of IgG with AcroSep Protein A Ceramic HyperD® F columns as compared to competitive columns.Average value is derived from duplicate readings.(Flow rate:0.2 mL/min for loading, 1 mL/min all other steps.)

 

High Purity Protein in a Single Step Using Native Conditions

High Purity Protein in a Single Step Using Native Conditions

M:Protein molecular weight marker
L:Sample loaded on the column
FT:Unbound/flow through W:Wash fraction
E:Eluate

 

Purification of Albumin From Human Plasma


Purification of Albumin From Human Plasma

Total protein (0.3 mL plasma) was bound to the Blue Trisacryl® M AcroSep column in 20 mM PBS, pH 7.2. Bound proteins were eluted with a gradient of 3 M NaCl in PBS.

 

High Purity Protein in a Single Step Using Native Conditions


High Purity Protein in a Single Step Using Native Conditions

M:Protein molecular weight marker
L:Sample loaded on the column
FT:Unbound/flow through
W:Wash fraction
E:Eluate
Part Number 描述 Pkg Price Qty
AcroSep 亲合纯化层析柱
20078-C001 Protein A Ceramic HyperD, 1 mL, Pearl 5/pkg Call
25896-C001 Blue Trisacryl M, 1 mL, Dark Blue 5/pkg Call
 

 


pall,颇尔,AcroSep 亲合层析柱
上一条pall,颇尔,用于裂解液澄清的AcroPrep Advance 孔板,8075, 8040, 8175, 8275
下一条pall,颇尔,AcroSep 离子交换层析柱,20050-C001, 20062-C001, 20066-C001, 20067-C001, IEXVP-C001

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