pall,颇尔,用于超滤的AcroPrep Advance 96 孔板,8033, 8034, 8035, 8036, 8163, 8164, 8165, 8166

pall,颇尔,用于超滤的AcroPrep Advance 96 孔板

Provides rapid, efficient separation of biomolecules

• Omega™ membrane provides high recovery and typically results in > 90% recovery of target biomolecules
• New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume for maximum sample recovery
• Intrinsic plate and membrane properties prevent target molecules from binding to the plate

• 分子排阻
• PCR clean-up
• 核酸纯化
• 蛋白分离

材料成分

• Filter Media：Omega (modified polyethersulfone) membrane
• Plate Housing:Polyproylene
• Lid:Polystyrene

 

尺寸

• Length:12.8 cm (5.0 in.)
• Width:8.6 cm (3.4 in.)
• Height With Lid:1.8 cm (0.7 in.) (350 μL only)
• Height Without Lid:350 μL, 1.4 cm (0.6 in.);1 mL:3.3 cm (1.3 in.)

 

Well-Bottom Area

• 0.25 cm²

 

Recommended Working Volume

• 350 μL: < 300 μL
• 1 mL: < 900 μL

 

Recommended Operating Vacuum

• > 25.4 cm Hg (10 in.Hg)

 

Recommended Centrifugal Force

• 1,500 x g

 

Typical Vacuum Filtration Performance

膜	Processing Time (min)	Hold-Up Volume (μL)
10K Omega	20	5
30K Omega	8	6
100K Omega	4	7

 

Typical Centrifugal Filtration Performance

膜	Processing Time (min)	Hold-Up Volume (μL)
3K Omega	45	2
10K Omega	8	2
30K Omega	8	2
100K Omega	5	2

Separation of Proteins with Omega Membrane Using Vacuum Filtration

SDS-PAGE analysis of reduced samples from 30K Omega membrane plate using vacuum separation method, 5 μL/lane.75 μL protein mix/well subjected to ~20 in.Hg vacuum for ~8 minutes.Each protein at 100 μg/mL in initial mix.ML = Marker Ladder; M1 = BSA; M2 = BSA + Cyt C; M3 = BSA, Carbonic Anydrase, and Cyto C; M4 = Carbonic Anhydrase.GelCode^♦ Blue stain.

Related Literature

• 应用说明：高效多孔滤板蛋白纯化方案
• 应用说明：原核培养中的改造质粒 DNA 的纯化

 

相关产品

• AcroPrep Advance Filter Plates for Multiplexing ensure assay reproducibility. 
• AcroPrep Advance Filter Plates for Neonatal Screening for consitent performance and accurate results.
• AcroPrep Advance Filter Plates for Protein Purification have a versatile format for multiple purification strategies.
• AcroPrep Advance Filter Plates for Lysate Clearance provide efficient clarification of highly particulated samples.
• AcroPrep Advance Filter Plates for DNA Purification yield high quality DNA.
• AcroPrep Advance Filter Plates for Solvent Filtration provide compatibility with harsh organics.
• AcroPrep Advance Filter Plates for Aqueous Filtration for fast processing with efficient removal of particulates.
• AcroPrep 384-well Filter Plates for superior performance in high throughput sample preparation applications.
• Centrifugal Devices concentrate and purify samples of < 50 μL to 60 mL with efficient recovery and low non-specific binding.
• Chromatography Resins simplify protein purification and fractionation for biomarker discovery, proteomics research and drug development.
• Vacuum Manifold fits AcroWell and AcroPrep filter plates tightly for vacuum filtration.

Part Number	描述	Pkg	Price	Qty
用于超滤的 AcroPrep Advance 滤板
8033	350 μL, Omega 3K MWCO	10/pkg	Call
8034	350 μL, Omega 10K MWCO	10/pkg	Call
8035	350 μL, Omega 30K MWCO	10/pkg	Call
8036	350 μL, Omega 100K MWCO	10/pkg	Call
8163	1 mL, Omega 3K MWCO	5/pkg	Call
8164	1 mL, Omega 10K MWCO	5/pkg	Call
8165	1 mL, Omega 30K MWCO	5/pkg	Call
8166	1 mL, Omega 100K MWCO	5/pkg	Call
配件及替换部件
5017	多孔板真空歧管	1/pkg	Call
5225	Adapter collar for centrifugation	2/pkg	Call
5226	Adapter for PCR receiver plate	2/pkg	Call
5230	Cap mat for incubation	5/pkg	Call
8001	AcroPrep Advance multi-well plate lids	10/pkg	Call

pall,颇尔,用于超滤的AcroPrep Advance 96 孔板