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颇尔,用于裂解液澄清的AcroPrep Advance 孔板
Prefilter Provides Efficient Clarification
of Highly Particulated Samples
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Integrated prefilter yields consistent filtration of samples with high levels of gross particulate
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New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume
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Innovative outlet tip geometry provides direct flow of samples into receiver plate without concerns of cross-contamination
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Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of target molecules
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Removal of bacterial debris prior to plasmid purification
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Removal of bacterial and cellular debris prior to protein purification
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Clearance of gross particulates
材料成分
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Filter Media:3 μm glass fiber/0.2 μm Supor® membrane and 3 μm glass fiber/1.2 μm Supor membrane
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Plate Housing:Polyproylene
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Lid:Polystyrene
尺寸
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Length:12.8 cm (5.0 in.)
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Width:8.6 cm (3.4 in.)
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Height With Lid:1.8 cm (0.7 in.) (350 μL only)
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Height Without Lid:350 μL, 1.4 cm (0.6 in.);1 mL:3.3 cm (1.3 in.);2 mL:4.7 cm (1.9 in.)
Well-Bottom Area
Recommended Working Volume
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350 μL: < 300 μL
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1 mL: < 900 μL
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2 mL: < 1.9 mL
Recommended Operating Vacuum
Recommended Centrifugal Force
Typical Processing Time
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PN 8040
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Vacuum:2 seconds
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Centrifuge:< 2 minutes
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PN 8075, 8175, 8275
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Vacuum:9 seconds
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Centrifuge:< 2 minutes
一般滞留量
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PN 8040 - Vacuum:17 μL
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PN 8075, 8175, 8275 - Vacuum:13 μL
Glass Fiber and Supor Membrane Allow Complete Recovery of Plasmid DNA from E. coli Lysates
Purified pCAT plasmid DNA was spiked in TE buffer at 25 μg/mL, 50 μg/mL, and 100 μg/mL and filtered through 颇尔 and Competitor A’s prefilter plates.Percent recovery was calculated by comparing OD260 post-filtration to a standard curve of unfiltered samples.
pall,
颇尔,用于裂解液澄清的AcroPrep Advance 孔板