pall,颇尔,Q, S, DEAE 和 CM Ceramic HyperD F 层析介质,20066-031, 20062-030, 20067-039, 20050-035, 20066-023, 20062-022, 20067-021, 20050-027, 20066-015, 20062-014, 20067-013, 20050-019, 20066-064, 20062-048,

pall,颇尔,Q, S, DEAE 和 CM Ceramic HyperD F 层析介质

简介

Q, S, DEAE and CM Ceramic HyperD® F ion exchange chromatography sorbents are designed using 颇尔’s proprietary 'gel-in-a-shell' design that provides rapid mass transfer and effective binding.It relies on a high capacity hydrogel, polymerized within the gigapores of a rigid ceramic bead.

特色及优点

• High dynamic binding capacity at high flow rates
• Truly rigid, non-compressible sorbent
• Salt tolerant CM Ceramic HyperD F sorbent reduces Ultrafiltration/Diafiltration (UF/DF) requirements

Sorbent Applications

• 重组蛋白
• 质粒纯化
• Purification of enzymes, plasma proteins
• 疫苗
• Direct capture of monoclonal antibodies (CM Ceramic HyperD® F)
• IgM

Sorbent Specifications

项目	Q Sorbent	S Sorbent	DEAE Sorbent	CM Sorbent
Average particle size (µm)	50	50	50	50
Dynamic Binding Capacity (mg/mL) 10% breakthrough at 200 cm/hr	BSA* > 85¹	lysozyme > 75²	BSA* > 85¹	IgG > 60³
Amount of ionic groups (µEq/mL)	> 250	> 150	> 200	250 - 400
Working pH	2 -12
清洁 pH	1 - 14
Pressure resistance	70 barg (1,000 psi)
Volumes changes due to pH and ionic strength	Non compressible

 

* BSA = Bovine Serum Albumin
¹ 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.6
² 5 mg/mL Lysozyme in 50 mM sodium acetate , pH 4.5
³ 5 mg/mL hu IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7

• Application Note “High Throughput Chromatography Sorbent Screening and Purification Process Optimization of a Fab Antibody Fragment Using AcroPrep™ ScreenExpert Plates and PRC Prepacked Columns”

Sorbent Ordering Information

	Ceramic HyperD F Sorbents
Pack Size	Q Sorbent	S Sorbent	DEAE Sorbent	CM Sorbent
25 毫升	20066-031	20062-030	20067-039	20050-035
100 毫升	20066-023	20062-022	20067-021	20050-027
1 L	20066-015	20062-014	20067-013	20050-019
5 L	20066-064	20062-048	20067-054	20050-050
10 L	20066-056	20062-055	20067-047	20050-043

 

Ceramic HyperD F Prepacked Chromatography Columns for Ion Exchange
PRC Prepacked Columns
Q Ceramic HyperD F 5x50, 1 mL, 1/pkg	PRC05X050QCHDF01
CM Ceramic HyperD F 5x50, 1 mL, 1/pkg	PRC05X050CMCHDF01
AcroSep Prepacked Columns
Q Ceramic HyperD F, 1 mL, Red, 5/pkg	20066-C001
S Ceramic HyperD F, 1 mL, Blue, 5/pkg	20062-C001
CM Ceramic HyperD F, 1 mL, Green, 5/pkg	20050-C001
DEAE Ceramic HyperD F, 1 mL, Orange, 5/pkg	20067-C001
(1) each:Q, S, CM, and DEAE Ceramic HyperD F, 1 mL, 4/pkg	IEXVP-C001

pall,颇尔,Q, S, DEAE 和 CM Ceramic HyperD F 层析介质