100-200 mm I.D. Chromatography
Columns for Pilot or Production Use
Resolute FM 柱特别适用于从工艺开发到生产规模的层析纯化，提供一系列常用介质。These glass and acrylic columns, for development and manufacturing applications, are robust and incorporate new features to improve the outcome of column packing events.应用于高效 Resolute DM 系列的业界公认的恒定压缩密封设计也纳入到 FM 系列中。The columns are ideal for use with 颇尔’s PKP process controllers providing an ideally suited platform for pilot scale and small volume manufacturing purification operations with a wide range of chromatographic media.Accessory column valves and tubing kits are available to provide a complete solution for your next purification challenge. View Resolute large scale process columns.
Key Features and Benefits
- 卫生设计
- 用于排除空气的倾斜结构
- 增加的调节能力可以减少人工操作的问题
- 灵活的从柱子顶到底的拆卸
- 对玻璃敏感的蛋白可以选择丙烯酸材质
Modular Design
Resolute FM columns can be packed and unpacked with the column adjuster remaining supported above the top of the tube.The central adjuster mechanism enables the top distribution cell to travel within the tube and to be raised clear of the tube for packing and unpacking operations.Thus eliminating the requirement to remove and lay down the adjuster and associated tubing. This improves operator safety and reduces preparation time with connected hoses being left in place.
The adjuster seal positioned close to the edge of the distribution plate is under constant compression within the tube.There is no need to adjust and fine tune seal compression sealing is assured every time without operator involvement.This feature avoids problems associated with seals being either under or over compressed.This avoids leakage of fluids past the seal during the lowering of the adjuster during axial bed compression.
Unlike other column designs, the adjuster is always maintained in a relative vertical plane to the column tube by the adjuster plate and does not need to be inserted by the operator at an angle into the tube to remove air while the seal is formed against the tube wall.Resolute FM columns have a unique feature on the lower frame which allows the column assembly to be tilted from vertical while the adjuster is lowered.This feature has simplified the packing preparation process and results in a reproducible outcome which can be defined in SOP’s and avoids awkward handling of heavy column components and attached tubing.This procedure is ideal for concise SOP’s and produces reproducible column priming outcomes due the security of the mechanical steps involved in place of manual manipulation of separate column parts.
Fast Packing and Unpacking
The design of the central adjuster mechanism provides clearance for entry of the slurry directly into the column tube.With a measured volume of slurry in the column, the liquid level is then topped up with buffer to a specific bed height (marked on the tube unit).The column is then set in the priming position and the adjuster lowered into the tube by rotating the centrally located handles.As the seals enter the tube the air is displaced, and once the seal is made the column is again set vertical before applying the required flow to pack the bed.Once the bed is compressed to a target value the flow is stopped and the adjuster lowered to achieve an optimal pack.
Unpacking can be performed through the bottom or top of the tube.Typically, the adjuster is raised by rotating the centrally located handles until it is clear of the tube.After rinsing the top distribution plate and net to recover residual media, the column contents are gently agitated with buffer to form a slurry before being pumped to a container.
General Specifications
| 产品 | Diameter (mm) | Adjustable Height Range (mm) | Adjustable Capacity Packed Volume (L)* | Maximum Allowable Pressure (barg/psig) |
| Resolute FM 100 | 100 | 100 - 460 | 0.78 - 2.10 | 5.0/72.5 |
| Resolute FM 140 | 140 | 100 - 460 | 1.54 - 4.10 | 5.0/72.5 |
| Resolute FM 200 | 200 | 100 - 460 | 3.14 - 8.40 | 4.6/66.7 |
| 产品 | Net Weight (kg/lb) | Column Height at Minimum Bed (mm) | Width (mm) | Depth (mm) | Height (mm) |
| Resolute FM 100 | 30/66 | 1105 | 385 | 385 | 1625 |
| Resolute FM 140 | 45/99 | 1135 | 415 | 415 | 1655 |
| Resolute FM 200 | 70/154 | 1135 | 455 | 455 | 1655 |
*Based on 70% slurry and compression factor x 1.2
Temperature
- 2 - 35 ºC
Column Termination Sizes
|
 |
|||||||||||||||||||
Component Weights Kg (lb)
| FM100 | FM140 | FM200 | |
| Column | 30.0 (66.1) | 46.0 (101.4) | 62.0 (136.7) |
| Adjuster Assembly Complete | 8.6 (19.0) | 18.6 (41.0) | 25.1 (55.3) |
Materials of Construction - Process Wetted Components
-
Tube:
- Glass, borosilicate 3.3
- 丙烯酸
-
Pipework / Distribution Plate
- Stainless Steel, grade 316 L (EN 1.4404)
-
Nets
- 20 μm – Polypropylene 10 μm – Polyamide
-
Net Support
- 聚丙烯
-
密封圈
- EPDM, black, peroxide cured
Materials of Construction - External Components
-
Tube
- Glass, borosilicate 3.3
- 丙烯酸
-
Seals:
- EPDM, black, peroxide cured
-
Pressure Envelope Components
- Stainless steel, grade 316 L (EN 1.4404)
- PEEK
-
Top Flange
- 聚丙烯
-
Stand
- Stainless steel, grade 316 L (EN 1.4404)
-
Castors
- Stainless steel, grade 304
-
Wheels
- Acetal
Materials used in the manufacture of the column product contacting components either conform to relevant sections of the FDA Code of Federal Regulations Volume 21, parts 170 to 199, or are non-FDA recognized materials that pass USP Class VI tests for toxicity.All Wetted Elastomers Conform to USP VI or CFR 177.2600.
Applicable Codes and Standards
| Design Standard: | |
| Steel Components | PD5500 (2012) |
| Acrylic tube | PED Material Appraisal |
| Mechanical Design | PED 97/23/EC |
| P.E.D. Category | SEP |
化学兼容性
The wetted materials of construction of Resolute FM are industry standard and are compatible with the majority of aqueous solutions used in the biotechnology industry.If you have queries about a particular application, please contact your local sales representative.图 1：Packed Bed Performance
Acetone 2% (w/v), injection volume 1-5% CV, bed height 20 cm.FM100
FM140
FM200
Sanitisation
Resolute FM columns are suitable for sanitization in empty or packed mode, as well as having easy access to all seals and supports for fast maintenance requirements.With a minimal number of parts, access for wipe down and the electropolished stainless steel surface finish minimizes entrapment of contaminants and reduces the risk of corrosion.Validation of all column sizes demonstrated that the columns can be effectively sanitized.
Chromatography Process Solutions from 颇尔
颇尔 offers a comprehensive, versatile and environmentally friendly range of chromatography sorbents that greatly simplifies protein purification challenges from lab to process scale.颇尔层析填料可提高下游工艺的纯化精度、速度、安全性、可靠性并降低纯化成本。Sorbent chemistries include ion exchange, mixed mode, hydroxyapatite, affinity, size exclusion, solvent-detergent removal and HIC.
The PK solution platform and PKP chromatography system range incorporate proven component technology, delivering accurate precision gradients as well as in-line buffer dilution.This occurs while maintaining the performance necessary to make your chromatography process consistent from batch-to-batch and optimal at pilot and manufacturing scales.Covering a range from 1 - 4000 L/h, 颇尔’s PK and PKP systems ensure robust operation and flexibility for all biochromatography applications.These systems can be used with Resolute chromatography columns as well as Mustang® chromatography membrane adsorber capsules.
颇尔 LRC chromatography columns have been designed to meet the requirements of most laboratory applications (ion exchange, affinity, size exclusion, mixed-mode chromatography).Four internal diameters, from 10 to 50 mm, and four lengths provide a range of column volumes up to 900 mL and bed heights up to 750 mm.Each column is supplied assembled and ready-to-use with tubing and fittings for connection to a standard system.The column body is made of a borosilicate glass tube.The columns are equipped with one adjustable and one fixed plunger, and a screw-lock system, to allow a rapid adjustment of the piston.
The Resolute DM columns are equipped with 颇尔’s proprietary high resolution mobile phase flow path, which consists of a mobile phase flow path within the nozzle valve body, distribution end cells, and a fully flushed piston seal arrangement.The performance of these essential column components is increasingly important for optimal process efficiency, capacity, and peak symmetry when scaling up to larger capacity columns.
Resolute DM columns maintain identical design parameters throughout both the standard column range (from 280 mm to 1200 mm diameter) and the larger capacity columns (up to 2000 mm diameter).Operation at high linear velocities is free from non-linear column effects and assures true linear scalability of column performance, enabling users to scale up simply without method redevelopment or impact on the reproducibility of the packing process.
Adjustable Capacity Columns
| Item Description |
Diameter (mm) |
Resolute FM, Glass | Resolute FM, Acrylic |
| FM 100/100-460 | 100 | FM100A100A20D | FM100AC100A20D |
| FM 140/100-460 | 140 | FM140A100A20D | FM140AC140A20D |
| FM 200/100-460 | 200 | FM200A100A20D | FM200AC200A20D |
注：Column ships with 20 μm nets fitted.
Spares Kits and Accessories
|
Column Ø (mm) |
Tube Kit, Glass | Tube Kit, Acrylic |
Fixed & Adjuster Net (10 μm) |
Fixed & Adjuster Net (20 μm) |
Adjuster Net Support |
Fixed Net Support1 |
Wetted Seals2 |
Non Wetted Seals3 |
| 100 | SFM100-A520A99 | SFM100-AC520A99 | SFM100-A150A99 | SFM100-A160A99 | SFM100-A350A99 | SFM100-A360A99 | SFM100-A300A99 | SFM100-A340A99 |
| 140 | SFM140-A520A99 | SFM140-AC520A99 | SFM140-A150A99 | SFM140-A160A99 | SFM140-A350A99 | SFM140-A360A99 | SFM140-A300A99 | SFM140-A340A99 |
| 200 | SFM200-A520A99 | SFM200-AC520A99 | SFM200-A150A99 | SFM200-A160A99 | SFM200-A350A99 | SFM200-A360A99 | SFM200-A300A99 | SFM200-A340A99 |
配件
| 配件 | Tools | |||||
|
Column Ø (mm) |
Column Valve Kit4 |
Bottom Valve Mounting Kit5 |
Foot Kit | Tubing Kit6 | Deep Socket7 | Stirring Paddle |
| 100 | SFM100-A270A99 | SFM100-A320A99 | SFM100-A310A99 | SFM100-A420A99 | SFM100-A330A99 | SFM100-A390A99 |
| 140 | SFM140-A270A99 | SFM140-A320A99 | SFM100-A310A99 | SFM140-A420A99 | SFM140-A330A99 | SFM100-A390A99 |
| 200 | SFM140-A270A99 | SFM140-A320A99 | SFM100-A310A99 | SFM140-A420A99 | SFM140-A330A99 | SFM100-A390A99 |
1.Net support (1) to fit between net and distributor
2.Adjuster seal (1), tube seal (2) , inlet tube seal (1)
3.Adjuster nut flange seal (1), inlet spool top seals (2)
4.Two way valve (1), clamp (1), gasket (1) [2 valve kits required per column for top and bottom]
5.Bottom elbow (1), clamp and gasket (1), bracket (1) and bolts/washers (2)
6.Reinforced tubing (2 x 150 cm, 1 x 200 cm) with hose barbs (6), gaskets (6) and clamps (6)
7.Required when dismantling tube assembly
Installation and Validation Documentation
- Each column is supplied with a user manual
-
A comprehensive validation and support package is also supplied, including:
- Setup and test report
- Materials and compatibility data
- Comprehensive parts list
