简介
SDR HyperD® solvent-detergent removal chromatography sorbent is a unique sorbent designed to eliminate solvent and detergent from biological fluids.It combines a silica-bead moiety filled with a three-dimensional cross-linked hydrophobic polymer. The particle size distribution (40-100 µm), the small pore size of the silica beads and the nature of the polymer have been optimized for a retention of solvents and detergents typically used in viral inactivation processes (i.e. Tri-n-Butyl Phosphate – TnBP – , Triton X-45 and Triton X-100).
The adsorption mechanism involves both the silica moiety and the hydrophobic polymer.The adsorption of Triton X-100 is proportional to the silica surface area, whereas the adsorption of TnBP is linked to binding to the organic polymer moiety. Large molecules are excluded.
特色及优点
- Efficient solvent and detergent removal from plasma fractions
- Easier to handle compared to conventional oil extraction or C18 chromatography methods
- Detergent removal from biological samples
- Rigid and mechanically stable for process scale
Sorbent Specifications
| Sorbent Structure | Spherical silica beads filled with a three-dimensional hydrophobic polymer |
| Average Particle Size | 40 - 100 微米 |
| Binding Capacity for Triton X100* | ≥90 mg/mL |
| Typical Sample Load | 2 – 3 times the column volume with residence times of5 min using IgG or ATIII treated solutions |
| Recommended Residence Time | 5 – 15 min |
| Operating pH Range | 2 – 12 |
| Sorbent Pressure Resistance | 70 bar (1000 psi) |
* Determined using 5 mg/mL Triton X-100 in PBS, pH 7.4, 10% breakthrough, 300 cm/h
Sorbent Ordering Information
| 尺寸 | SDR HyperD 填料 |
| 25 毫升 | 20033-031 |
| 100 毫升 | 20033-023 |
| 1 L | 20033-015 |
| 5 L | 20033-056 |
| 10 L | 20033-049 |
