pall,颇尔,PRC预装柱,预装Q和CM Ceramic HyperD 离子交换层析介质,PRC05X050QCHDF01, PRC05X050CMCHDF01

产品名称:
PRC预装柱,预装Q和CM Ceramic HyperD 离子交换层析介质
产品型号:
PRC05X050QCHDF01, PRC05X050CMCHDF01
产品描述:

产品介绍

pall,颇尔,PRC预装柱,预装Q和CM Ceramic HyperD 离子交换层析介质,PRC05X050QCHDF01, PRC05X050CMCHDF01
pall,颇尔,PRC预装柱,预装Q和CM Ceramic HyperD 离子交换层析介质

PRC<a href='http://pall.net.cn/' target='_blank'>预装柱</a>,预装Q和CM Ceramic HyperD 离子交换层析介质
Rapid selectivity screening of Ceramic HyperD ion exchangers
  • Convenient – Ready-to-use 1 mL prepacked columns.
  • Easy to use – Direct connection to commonly-used laboratory chromatography systems such as ÄKTA* systems.
  • Efficient – High packing efficiency (> 2,500 plates/meter).
  • Consistent – Screen 颇尔 ion exchange chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

离子交换层析介质

Q and CM Ceramic HyperD sorbents have a good capacity hydrogel polymerized within the gigapores of a rigid ceramic bead.Typical features include:
  • Good dynamic binding capacity independent of flow rate
  • Allowing a direct capture with high binding capacity for IgG at conductivities of 10 to 15 mS/cm on CM Ceramic HyperD sorbent

PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

*ÄKTA is a trademark of GE Healthcare companies.
 
 
1 毫升 PRC 柱的快速筛选和条件优化可快速选择合适的化学成分。

可以将两个或两个以上柱串联起来以增加柱高,并更真实地模拟中试规模或按比例缩减规模应用的现实条件。Further scale up can be achieved with minimal re-optimization by packing 颇尔 LRC empty glass columns.

离子交换层析介质

  • Native and recombinant proteins
  • Plasmids
  • 疫苗
  • Monoclonal and polyclonal antibodies
  • 血液制品
  • 生物制药
 
 

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)

 

Functionalities
Ion exchange:Q and CM Ceramic HyperD F sorbents
 

Storage Solution
20% ethanol/150 mM NaCl

Working Pressure1
< 1.5 barg (22 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns

材料成分
Body and end caps:Molded polypropylene
17 µm frit:PP/PE (Polypropylene/Polyethylene)

Connections2
Built-in 10-32 fittings

最大操作压力
20 barg (290 psig) for 1 mL columns

1 Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
2 For HPLC/MPLC/AKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings.For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

 

 
填料   Average Particle Size (µm) Ionizable Groups (µEq/mL) Dynamic Capacity (mg/mL)1
离子交换 Chemistry       
Q 陶瓷 HyperD F Quaternary amine 50 > 250 > 852
CM Ceramic HyperD F 50 > 250 > 603
 

 

1 Determined at 10% breakthrough using:
2 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h
3 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h
 
 

Separation of Cytochrome C, BSA and Human Transferrin on a 颇尔 PRC Q Ceramic HyperD F Prepacked Column

Column:颇尔 PRC Column 5x50 Q Ceramic HyperD F, 5 mm I.D. x 50 mm, volume:1 mL, linear flow rate:150 cm/h.Equilibration and wash:50 mM Tris-HCl, pH 8.6. Load:100 µL (5 mg/mL cytochrome C, 20 mg/mL BSA and 20 mg/mL human transferrin in equilibration buffer).Elution:50 mM Tris-HCl, pH 8.6 + 0.5 M NaCl by linear gradient from 0 to 100% in 60 minutes.
 

颇尔 offers a comprehensive line of chromatography columns from lab to process scale.

 

 PRC 预装柱

PRC Columns
1 - 5 mL (prepacked)
Fast selectivity screening

 LRC 层析柱

LRC Columns
5 - 900 mL
Preparative and process optimization
 Resolute Columns

Resolute® Columns
6 - 300 L
Pilot and small-scale production

 Resolute Columns

Resolute Columns
50 - 1500 L
Large-scale production

 

 
Part Number 描述 Volume
PRC05X050QCHDF01 PRC Column 5x50 Q Ceramic HyperD F 1 毫升
PRC05X050CMCHDF01 PRC Column 5x50 CM Ceramic HyperD F 1 毫升
 

 


pall,颇尔,PRC预装柱,预装Q和CM Ceramic HyperD 离子交换层析介质
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