- Convenient – Ready-to-use 1 mL prepacked columns.
- Easy to use – Direct connection to commonly-used laboratory chromatography systems such as ÄKTA* systems.
- Efficient – High packing efficiency (> 2,500 plates/meter).
- Consistent – Screen 颇尔 ion exchange chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.
离子交换层析介质
Q and CM Ceramic HyperD sorbents have a good capacity hydrogel polymerized within the gigapores of a rigid ceramic bead.Typical features include:- Good dynamic binding capacity independent of flow rate
- Allowing a direct capture with high binding capacity for IgG at conductivities of 10 to 15 mS/cm on CM Ceramic HyperD sorbent
PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.
*ÄKTA is a trademark of GE Healthcare companies.
可以将两个或两个以上柱串联起来以增加柱高,并更真实地模拟中试规模或按比例缩减规模应用的现实条件。Further scale up can be achieved with minimal re-optimization by packing 颇尔 LRC empty glass columns.
离子交换层析介质
- Native and recombinant proteins
- Plasmids
- 疫苗
- Monoclonal and polyclonal antibodies
- 血液制品
- 生物制药
Sorbent Specifications
Volume1 mL (5 mm ID x 50 mm)
Functionalities
Ion exchange:Q and CM Ceramic HyperD F sorbents
Storage Solution
20% ethanol/150 mM NaCl
Working Pressure1
< 1.5 barg (22 psig)
Column Specifications
Outer Dimensions10 x 100 mm for 1 mL columns
材料成分
Body and end caps:Molded polypropylene
17 µm frit:PP/PE (Polypropylene/Polyethylene)
Connections2
Built-in 10-32 fittings
最大操作压力
20 barg (290 psig) for 1 mL columns
1 Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
2 For HPLC/MPLC/AKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings.For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.
填料 | Average Particle Size (µm) | Ionizable Groups (µEq/mL) | Dynamic Capacity (mg/mL)1 | |
离子交换 | Chemistry | |||
Q 陶瓷 HyperD F | Quaternary amine | 50 | > 250 | > 852 |
CM Ceramic HyperD F | 羧 | 50 | > 250 | > 603 |
1 Determined at 10% breakthrough using:
2 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h
3 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h
Separation of Cytochrome C, BSA and Human Transferrin on a 颇尔 PRC Q Ceramic HyperD F Prepacked Column
Column:颇尔 PRC Column 5x50 Q Ceramic HyperD F, 5 mm I.D. x 50 mm, volume:1 mL, linear flow rate:150 cm/h.Equilibration and wash:50 mM Tris-HCl, pH 8.6. Load:100 µL (5 mg/mL cytochrome C, 20 mg/mL BSA and 20 mg/mL human transferrin in equilibration buffer).Elution:50 mM Tris-HCl, pH 8.6 + 0.5 M NaCl by linear gradient from 0 to 100% in 60 minutes.
颇尔 offers a comprehensive line of chromatography columns from lab to process scale.
PRC Columns |
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LRC Columns 5 - 900 mL Preparative and process optimization |
Resolute® Columns |
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Resolute Columns |
Part Number | 描述 | Volume |
PRC05X050QCHDF01 | PRC Column 5x50 Q Ceramic HyperD F | 1 毫升 |
PRC05X050CMCHDF01 | PRC Column 5x50 CM Ceramic HyperD F | 1 毫升 |